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. 2017 Aug 17;22(8):1363. doi: 10.3390/molecules22081363

Figure 3.

Figure 3

SM70EE prevents neuronal cell damage through enhancing BDNF/CREB signaling pathways and suppressing AChE activity against TMT-induced neurotoxicity in HT-22 cells. (A) Proteins were harvested at the indicated time points and subjected to western blot analysis to probe the expressions of p-CREB, p-TrkB; (B) Proteins were harvested at the indicated time points and subjected to western blot to analyze to the expression of BDNF. The protein expression level was quantified using Image J software and normalized against α-tubulin and control; (C) HT-22 cells were pre-treated 50 and 100 μg/mL SM70EE for 4 h before treatment with 10 μM TMT for 24 h. AChE activity was measured by Ellman method. The experiments were performed in tri-plicate. Results were analyzed by one-way ANOVA and Duncan’s multiple range tests. The p-value in the multiple comparison results (e.g., a, b, c, and d) indicate significant differences among the groups (p < 0.05).