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. 2017 Jun 1;22(6):918. doi: 10.3390/molecules22060918

Table 2.

Linear regression, LOD, LOQ and precision of 40 standard compounds and stability and recovery of the analytes in a sample solution.

No. b Regression Equation c r2 Linear Range (µg/mL) LOD (µg/mL) LOQ (µg/mL) Precision (RSD, %) Stability (RSD, %) Recovery (n = 6)
Intraday (n = 6) Interday (n = 18) Mean, % RSD, %
1 y = 392.6x − 49.916 0.9954 0.524–26.2 0.0586 0.1953 2.97 3.99 3.98 102.5 3.19
2 y = 630x + 57.152 0.9948 0.616–30.8 0.0177 0.0588 2.77 4.93 4.85 99.9 1.29
3 y = 180160x + 97682 0.9970 0.425–21.3 0.0001 0.0004 2.16 4.14 4.02 102.6 4.81
4 y = 11809x + 4671.1 0.9952 0.452–22.6 0.0018 0.0061 2.41 4.08 4.76 103.8 3.08
5 y = 363.6x + 120.5 0.9995 0.472–23.6 0.0611 0.2036 2.97 4.8 4.19 104.6 1.32
6 y = 3139.2x + 2525 0.9963 0.472–23.6 0.0068 0.0227 2.41 4.53 4.59 102.7 3.89
7 y = 141382x + 94037 0.9974 0.560–28.0 0.0002 0.0005 3.09 3.65 4.05 104.9 2.50
8 y = 3774.2x + 1124.3 0.9998 0.508–25.4 0.0059 0.0197 2.57 4.62 4.27 103.1 4.08
9 y = 4960.3x + 4375.9 0.9965 0.572–28.6 0.0045 0.0149 2.23 4.49 4.24 104.2 2.35
10 y = 5863.7x + 298.23 0.9999 0.540–27.0 0.0038 0.0128 3.15 3.87 3.81 104.4 3.95
11 y = 891.7x + 849.87 0.9991 0.480–24.0 0.0245 0.0817 3.09 4.09 4.17 103.5 3.74
12 y = 905.2x + 781.29 0.9991 0.476–23.8 0.0242 0.0807 3.92 4.2 4.44 97.8 1.20
13 y = 187.1x + 11.88 0.9933 0.472–23.6 0.1181 0.3933 3.45 4.12 4.81 96.4 4.59
14 y = 961.0x − 72.951 0.9948 0.496–24.8 0.0243 0.0809 2.34 4.17 4.28 95.2 2.78
15 y = 210.9x − 44.971 0.9980 0.480–24.0 0.1088 0.3623 2.89 3.09 3.24 102 3.11
16 y = 338x − 21.961 0.9968 0.447–22.4 0.0668 0.2224 3.02 3.45 3.66 98.6 2.82
17 y = 618x − 5.3866 0.9996 0.424–21.2 0.0364 0.1213 3.22 3.4 3.27 105.7 1.62
18 y = 735x − 23.523 0.9967 0.480–24.0 0.0315 0.1048 3.16 3.78 4.03 104.5 3.80
19 y = 3232.4x + 849.76 0.9992 0.584–29.2 0.0068 0.0227 3.23 4.85 4.33 97.7 3.16
20 y = 11236x − 5692 0.9967 0.588–29.4 0.0020 0.0067 2.18 4.34 4.68 99.8 1.01
21 y = 2574.4x + 2231.2 0.9994 0.620–31.0 0.0086 0.0285 3.2 3.75 3.61 97.5 4.05
22 y = 5387.7x − 845.81 0.9957 0.504–25.2 0.0041 0.0138 2.09 3.63 3.73 101.1 3.41
23 y = 135.8x + 173.86 0.9958 0.628–31.4 0.1607 0.5352 2.62 3.58 3.18 95.5 4.88
24 y = 402.8x + 122.7 0.9989 0.488–24.4 0.0559 0.186 2.31 3.87 3.94 98.9 2.49
25 y = 548.0x − 5.2532 0.9970 0.504–25.2 0.0419 0.1394 3.48 4.24 4.26 98.2 3.08
26 y = 283.7x + 154.16 0.9994 0.744–37.2 0.0789 0.2627 2.34 3.76 3.45 97.2 3.31
27 y = 860x − 3.1856 0.9992 0.700–35.0 0.0263 0.0875 2.76 3.65 3.45 100.5 2.62
28 y = 1042x + 83.479 0.9980 0.752–37.6 0.0212 0.0705 2.64 4.13 2.16 100.0 1.17
29 y =518x + 31.816 0.9980 0.588–29.4 0.04308 0.1435 2.81 4.42 4.38 99.3 4.94
30 y = 136x + 100.17 0.9999 0.708–35.4 0.1625 0.5412 3.23 4.87 4.91 103.2 1.69
31 y = 861x + 98.616 0.9962 0.524–26.2 0.0253 0.0843 2.59 3.9 3.55 98 3.40
32 y = 539x − 15.987 0.9973 0.628–31.4 0.0416 0.1385 2.59 3.33 3.75 97.5 2.71
33 y = 295.7x + 241.23 0.9972 0.592–29.6 0.0749 0.2494 2.38 3.63 3.52 102.5 2.08
34 y = 550.3x + 519.33 0.9901 0.640–32.0 0.0412 0.1373 2.66 4.79 4.40 105.4 2.92
35 y = 780x + 24.97 0.9984 0.816–40.8 0.0272 0.0905 2.75 4.78 4.18 97.4 2.88
36 y = 940x − 21.517 0.9999 0.704–35.2 0.0178 0.0592 2.9 4.39 4.83 96.2 3.80
37 y = 368x − 19.204 0.9985 0.596–29.8 0.0624 0.2079 2.79 4.71 4.73 97.7 3.56
38 y = 465x + 432.16 0.9979 0.560–28.0 0.0465 0.1548 2.94 3.89 3.96 98.5 4.06
39 y = 266.5x + 5.1122 1.000 0.720–36.0 0.0845 0.2813 2.15 3.12 3.27 96.5 3.13
40 y = 162x − 17.799 0.9999 0.492–24.6 0.1457 0.4852 2.99 3.24 3.41 105.0 4.00

a The sample was prepared from No. 1; b The 40 analytes was the same as in Table 1; c x is the concentration of each compound in µg/mL; y is the peak area of the respective compound.