Figure 3.

Distribution of excitatory and inhibitory presynaptic terminals onto PV⁺ INs in P28 Mecp2 KO mice. Representative confocal images of excitatory, VGLUT1⁺ (green: A, C) and inhibitory, VGAT⁺ (blue: B, D) synaptic terminals contacting PV⁺ (red) dendrites (top) and somata (bottom) in layer II/III of S1 cortex in P28 WT and Mecp2 KO mice. Histograms showing quantitative analysis in WT and Mecp2 KO mice of VGLUT1⁺ and VGAT⁺ puncta density contacting either dendrites (E, F) or somata (G, H), respectively, of PV⁺ INs. Representative confocal images of VGLUT1⁺ (green: I, K) and VGAT⁺ (blue: J, L) puncta contacting CR⁺ (red) dendrites (top) and somata (bottom) in layer II/III of S1 cortex in P28 Mecp2 KO mice and WT littermates. Histograms showing quantitative analysis in WT and Mecp2 KO mice of VGLUT1⁺ and VGAT⁺ puncta density contacting either dendrites (M, N) or somata (O, P), respectively, of CR⁺ INs. PV: GLUT1 dendrites n = 5 mice per genotype; GLUT1 soma n = 8 mice per genotype; VGAT n = 5 mice per genotype; CR: VGLUT1 and VGAT n = 5 mice per genotype. Student’s t test: **p < 0.01. Scale bars = 5 μm.