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. 2017 Mar 23;22(4):506. doi: 10.3390/molecules22040506

Figure 4.

Figure 4

Anti-inflammatory activity study of compounds 1 and 2. Cells were treated with compounds 1 or 2 for 1 h and then co-incubated with 1 μg/mL LPS for 18 h. The NO production was determined by Griess assay (a) and the release of prostaglandin E2 (PGE2) was measured by ELISA assay (b); RAW264.7 cells were pretreated with compounds 1 or 2 and then incubated with LPS (1 μg/mL) for 18 h. Western blot assay was used to detect iNOS (c) and COX-2 protein expression (d). ### p < 0.001, compared to the control group; * p < 0.05, ** p < 0.01, *** p < 0.001, compared to the LPS-treated group. GAPDH: glyceraldehyde-3-phosphate dehydrogenase.