Fig. 2.
Multifarious histological features of SRRM4-transduced prostate cancer xenografts. DU145, PC-3, LNCaP, and 22Rv1 stable cell lines overexpressing SRRM4 or its respective control cells were inoculated in bilateral flanks subcutaneously in 6–8-week-old nude mice (n = 3 mice per cell line). Mice were euthanized when tumor burdens reached experimental endpoint (volume of 1500 mm3) and the tumors were harvested, fixed, paraffin embedded, and cut for IHC detection against (a) E-cad, pan-cytokeratin, SYP, and (b-c) Ki-67. (c) Previously processed NCI-H660 xenografts were also cut for IHC detection against Ki-67. Ki-67 was quantified by using the Aperio Image Analysis IHC menu (Leica Biosystems) and the results are represented on the dot plot. Results are presented as the mean ± SD (One-way ANOVA; n = 5; ***denotes p <0.001). Scale bars represent 100 μm. IHC, immunohistochemistry; E-Cad, E-cadherin; SYP, synaptophysin.