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Induction of apoptosis in Caco2 cells by 2-hydroxymelatonin, as determined by staining with Annexin V-FITC and propidium iodide (PI). (a) Annexin V-FITC and PI staining of Caco2 cells treated with 1 mM melatonin or 2-hydroxymelatonin; (b) Quantification of apoptosis induction in cells treated with 1 mM melatonin or 2-hydroxymelatonin. The results are representative of three experiments. EtOH, ethanol; M, melatonin; 2-OH-M, 2-hydroxymelatonin.
