FIG 6 .
Transformation of B. subtilis 168 amyE::Pxyl-comK with DyLight 650-labeled thrC::Pspank-parB-gfp DNA. On average, foci became visible after 6 h and 45 min. Fifteen percent of the cells expressed gfp or produced daughter cells expressing gfp. The arrows indicate a selection of foci within cells expressing gfp. Imaging occurred every 15 min. Imaging details are as follows: 100× phase-contrast oil lens; size, 640 by 640; pixel size, 0.06430 0.06430 0.200; bin, 1×1; Cy5 exp, 0.8 s; ND, 32%. GFP exp, 0.8 s; ND, 32%.