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. 2018 Sep 6;159(10):3581–3595. doi: 10.1210/en.2018-00559

Figure 5.

Figure 5.

TRPS1 regulates cell proliferation, which is PR-dependent. (A) T47D cells were transfected with either control (siC) or TRPS1 siRNA. After transfection, cells were treated with VC, 10 nM R5020, 1 μM TPA, or R5020+TPA for 24 hours and subjected to BrdU assay. The data are represented as fold change of VC treatment (dotted red line), mean ± SEM from three independent experiments. *P < 0.05; **P < 0.001. (B) PR levels were measured in CRISPR sr-c and PR knockout (PR-KO) clone #6 with immunofluorescent staining of PR (green) and DAPI (blue) to visualize the nuclei. Immunoblotting was done to monitor levels of PR in the sr-c and PR-KO cells upon treatment with VC, 10 nM R5020, 1 μM TPA, or R5020+TPA for 24 hours. Successful KO of PR is shown. (C) CRISPR sr-c and PR-KO clones #6, #13, and #15 were treated with VC, 10 nM R5020, 1 μM TPA, or R5020+TPA for 24 hours and subjected to BrdU assay to measure proliferation. The data are represented as fold change of sr-c VC treatment (dotted red line), mean ± SEM from three independent experiments. *****P < 0.0001. (D) Working model demonstrates a possible mechanism of TPA on PR action.