Fig. 4.
(a) Trapping of a 16 nt DNA from cis (up panel) or trans (bottom) while lowering the pH at the trans or cis side in the aerolysin pore. (b) The representative single channel current trace showing that the activated aerolysin pore can capture a 16 nt DNA with high sensitivity even at 10 mV with a prolonged translocation time. (c) Voltage-dependence of the DNA capture rate (kon) (left) and block duration (τ, right) at various acidic trans pH. (d) Discriminating DNA of different lengths (5-, 16- and 30 nt) from their block durations. Reprinted with permission from DOI: 10.1021/acs.analchem.7b03979, Anal. Chem., 2017.