Table 2.
Matrix effects and recoveries of CGA, NCGA, CCGA, CA, CA-3-G, CA-4-G, FA, and FA-d3 (IS) using six different rat plasma (n = 6).
| Compounds | Nominal Concentration (ng/mL) | Matrix Effect a (%) | Recovery b (Mean ± SD, %) |
|
|---|---|---|---|---|
| Mean | CV (%) | |||
| CGA | 1.5 | 91.6 | 7.6 | 63.0 ± 6.3 |
| 25 | 92.0 | 11.0 | 71.7 ± 6.9 | |
| 160 | 87.1 | 5.4 | 71.9 ± 9.4 | |
| NCGA | 1.5 | 96.1 | 3.6 | 41.4 ± 4.0 |
| 25 | 90.8 | 3.2 | 43.4 ± 3.7 | |
| 160 | 89.0 | 7.1 | 41.8 ± 1.4 | |
| CCGA | 7.5 | 98.0 | 6.6 | 61.5 ± 5.2 |
| 125 | 92.4 | 2.2 | 63.0 ± 7.8 | |
| 800 | 89.3 | 6.2 | 65.2 ± 5.7 | |
| CA | 7.5 | 87.7 | 7.2 | 94.0 ± 9.9 |
| 125 | 93.0 | 11.8 | 86.1 ± 10.4 | |
| 800 | 88.6 | 6.2 | 91.2 ± 6.3 | |
| CA-3-G | 7.5 | 95.9 | 2.0 | 46.5 ± 4.3 |
| 125 | 95.8 | 7.4 | 51.2 ± 3.4 | |
| 800 | 88.5 | 8.5 | 52.8 ± 4.2 | |
| CA-4-G | 7.5 | 92.7 | 1.7 | 51.3 ± 6.0 |
| 125 | 95.6 | 5.3 | 53.0 ± 3.4 | |
| 800 | 86.5 | 9.7 | 55.8 ± 4.2 | |
| FA | 37.5 | 96.8 | 2.2 | 93.3 ± 8.7 |
| 625 | 87.5 | 8.4 | 96.9 ± 8.7 | |
| 4000 | 97.5 | 8.6 | 97.4 ± 4.2 | |
| FA-d3 | 20 | 96.0 | 5.4 | 100.1 ± 2.0 |
a Matrix effect expressed as the ratio of the mean peak area of an analyte spiked post-extraction to the mean peak area of same analyte standards multiplied by 100. b Recovery calculated as the ratio of the mean peak area of an analyte–spiked plasma prior to liquid–liquid extraction to the mean peak of an analyte spiked after liquid-liquid extraction of blank plasma multiplied by 100.