Table 3.
Post-preparation, short-term, long-term and freeze-thaw stabilities of CGA, NCGA, CCGA, CA, CA-3-G, CA-4-G, and FA in rat plasma quality control samples (n = 3).
| Analytes and Nominal Concentration (ng/mL) | Post-Preparative (24 h at 4 °C) |
Short-Term (2 h on ice) |
Long-Term (28 days at −80 °C) |
Freeze-Thaw 3 Cycles (−80 °C to room temp.) |
||||
|---|---|---|---|---|---|---|---|---|
| CV, % | RE, % | CV, % | RE, % | CV, % | RE, % | CV, % | RE, % | |
| CGA | ||||||||
| 1.5 | 8.8 | −9.3 | 2.3 | −12.0 | 7.0 | −14.0 | 6.2 | −14.0 |
| 160 | 2.1 | 9.4 | 2.5 | −5.4 | 4.1 | −12.3 | 3.2 | −10.0 |
| NCGA | ||||||||
| 1.5 | 14.4 | −2.7 | 2.3 | −12.0 | 3.7 | −9.3 | 10.1 | −14.0 |
| 160 | 2.7 | 11.2 | 4.2 | −5.9 | 5.6 | −6.3 | 5.9 | −5.3 |
| CCGA | ||||||||
| 7.5 | 5.1 | −3.2 | 3.7 | −14.3 | 5.9 | −8.9 | 7.6 | −11.9 |
| 800 | 1.3 | 5.5 | 2.9 | −4.6 | 4.4 | −14.4 | 3.0 | −9.4 |
| CA | ||||||||
| 7.5 | 4.1 | −0.1 | 9.0 | −12.4 | 7.3 | −6.3 | 8.3 | −7.2 |
| 800 | 0.9 | 10.6 | 2.1 | −7.7 | 7.6 | −12.7 | 2.1 | −11.0 |
| CA-3-G | ||||||||
| 7.5 | 9.9 | −10.0 | 6.1 | −14.6 | 7.3 | −7.1 | 9.1 | −10.5 |
| 800 | 1.1 | 9.1 | 2.8 | −1.5 | 2.2 | −14.8 | 4.4 | −11.9 |
| CA-4-G | ||||||||
| 7.5 | 6.2 | −9.6 | 8.1 | −9.3 | 7.9 | 1.6 | 4.7 | −3.6 |
| 800 | 1.0 | 10.9 | 3.2 | −0.4 | 2.1 | −13.9 | 4.7 | −9.4 |
| FA | ||||||||
| 37.5 | 3.2 | −7.0 | 5.4 | −11.2 | 5.9 | −11.0 | 5.6 | −7.7 |
| 4000 | 4.6 | −8.6 | 2.7 | −4.8 | 6.1 | −8.8 | 3.9 | −8.4 |