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The formation of osthenol metabolites in human recombinant cDNA-expressed cytochrome P450 (CYP) isoforms (a–d) or cDNA-expressed uridine 5′-diphospho-glucuronosyltransferase (UGT) isoforms (e). Osthenol (20 μM) was incubated with each enzyme (5 pmole) for 60 min at 37 °C in the presence of a NADPH-regenerating system and 5 mM uridine 5′-diphosphoglucuronic acid. Data are expressed as the means ± SE of three independent determinations.
