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Particle size and EE variation after active loading of DOX at 60 °C. A significant changes were observed in particle sizes of EL and OA loaded liposome (OAL) (p < 0.01 (**) and p < 0.001 (###), respectively), before and after loading of DOX, while no significant change (ns) was observed in the EE of OA. Similarly, no significant change in EE was observed in the individual loading of both drugs in the liposome.
