Figure 2.

Step I: Connection of the IRGs and the TFs

(A) Distributions of EC₅₀ (upper) and T_1/2 (lower) values estimated for the upregulated IRGs (red bars) and the downregulated IRGs (blue bars). The dashed lines indicate the thresholds of the bimodal distributions.

(B) Time courses of the IRGs in each class classified by the EC₅₀ and T_1/2 values of the IRGs with high or low sensitivity and with fast or slow time constant. Blue, green, and red bold lines represent the averaged responses to 0.01, 1, and 100 nM insulin, respectively. Dashed lines indicate the time series of each IRG in the class. The y axis indicates the base 2 logarithm of fold change against expression of each gene at time 0 (log₂FC).

(C) The TFs predicted for each class of IRGs. Gray dashed arrows indicate the transcriptional regulation by the TFs, and red dashed arrows indicate that the Class 3 IRGs encode the TFs. The color code of the class is the same as in (B). The colored TFs are encoded by TFs of the matching class in Figure 3B: Creb1, Foxo, Foxo1A encoded by Class 1 TF; Hes1 and Egr1 encoded by Class 3 TFs.

See also Figure S1, and Tables S1, S2, and S3.