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. 2018 Sep 14;10(9):1305. doi: 10.3390/nu10091305

Figure 3.

Figure 3

BPF increases β-catenin and RUNX2 and decreases RANKL protein levels on Saos-2 cells. Semi-confluent cultures of human osteoblast-like cells (Saos-2) were incubated with BPF 0.001-0.01-0.1 mg/mL for 24 h. Cell proteins were analyzed by Western blotting (D) with antibodies specific to (A) β-catenin, (B) RUNX2, (C) RANKL and β-actin. Data are represented as mean ± SD. Statistical analysis: Student’s t-test vs. 0 * p < 0.05; ** p < 0.01. Linear regression # p < 0.05; ## p < 0.01. Abbreviations: BPF, Bergamot Polyphenol Fraction; RUNX2, Runt-related transcription factor 2; RANKL, Receptor Activator of Nuclear factor-κB Ligand.