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. 2018 Jul 16;8(3):54. doi: 10.3390/biom8030054

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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tRFs promote cardiac hypertrophy in cultured cardiomyocytes. (A) The distribution of different lengths of tRFs derived from tRNA-Gly-CCC; (B) Verification of the expression of tRFs by qRT-PCR, n = 3; (C) Transfection efficiency of tRFs mimics in the H9c2 cells, n = 3; (D) Relative mRNA expression of ANP, BNP, and β-MHC in treated H9c2 cells, n = 3; (E) Immunofluorescence evaluation of cardiac hypertrophy in cells treated with tRFs mimics. Bar indicates 50 μm, n = 3; (F) The relative cell surface area in treated H9c2 cells, n = 3; (G) Sequence alignment of tRFs1 with 3′-UTR of Timp3; (H) The repressive effect of tRFs1 on the activity of site1 of Timp3 3′UTR measured by luciferase assay, n = 3. (I) The repressive effect of tRFs1 on the activity of site2 of Timp3 3′UTR measured by luciferase assay, n = 3. Data are means ± SD. * p < 0.05, ** p < 0.01.

tRFs promote cardiac hypertrophy in cultured cardiomyocytes. (A) The distribution of different lengths of tRFs derived from tRNA-Gly-CCC; (B) Verification of the expression of tRFs by qRT-PCR, n = 3; (C) Transfection efficiency of tRFs mimics in the H9c2 cells, n = 3; (D) Relative mRNA expression of ANP, BNP, and β-MHC in treated H9c2 cells, n = 3; (E) Immunofluorescence evaluation of cardiac hypertrophy in cells treated with tRFs mimics. Bar indicates 50 μm, n = 3; (F) The relative cell surface area in treated H9c2 cells, n = 3; (G) Sequence alignment of tRFs1 with 3′-UTR of Timp3; (H) The repressive effect of tRFs1 on the activity of site1 of Timp3 3′UTR measured by luciferase assay, n = 3. (I) The repressive effect of tRFs1 on the activity of site2 of Timp3 3′UTR measured by luciferase assay, n = 3. Data are means ± SD. * p < 0.05, ** p < 0.01.