Figure 1.

Phenotypes of elp3 and sit4 mutants and their responses to overexpression of tRNA^GlnUUG, tRNA^LysUUU and tRNA^GluUUC. (A) BY4741 wild type (WT), elp3 or sit4 mutants were each transformed with empty plasmid pRS425 or pQKE (over-expression of tRNA^GlnUUG, tRNA^LysUUU and tRNA^GluUUC) and subjected to drop dilution spot assays involving yeast extract peptone (YP) based media with either glucose, galactose or ethanol as the carbon source. All plates were incubated at 30 °C for 3 days. (B) The same strains as in (A) were subjected to drop dilution spot assays on yeast extract peptone dextrose (YPD) medium (carbon source glucose) cultivated at 39 °C or 40 °C or on YPD medium supplemented with a crude zymocin preparation. (C) Microtiter based measurement of growth inhibition by soraphen A. Relative growth refers to optical density (O.D 600 nm) of microcultures with indicated concentrations of soraphen A as compared to soraphen A free cultures. Measurements were carried out in triplicate and presented as mean with standard deviation.