Figure 1.

SGT-53 increases immunogenicity and induces ICD. (A) 4T1 cells were treated with either SGT-53 or scL-vec nanocomplex. Expression of human p53 was assessed by quantitative RT-PCR. The fold-change relative to mouse GAPDH mRNA is shown on a log scale (n = 6). (B) Expression of mouse genes associated with immune responses was assessed by RT-PCR in the cells treated with SGT-53 (n = 6). (C) Release of HMGB1 and ATP was assessed in the culture media (n = 6). (D) Induction of apoptosis was assessed via Annexin V/7-AAD staining at 48 h after transfection. Numbers in the quadrants indicate the percentage of cells in that quadrant. (E) Expression of cell surface components of immunogenicity was assayed at 48 h after transfection via FACS (n = 4). Data are shown as mean ± SEM. *p < 0.001, **p < 0.05, 1-way ANOVA with Bonferroni t-test.