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. 2016 Jun 9;13(3):297–303. doi: 10.1007/s13770-016-9054-9

Local injection of granulocyte-colony stimulating factor accelerates wound healing in a rat excisional wound model

Guang-Yin Shen 1, In-Hwa Park 2, Yi-Sun Song 2, Hyun-Woo Joo 2, Yonggu Lee 1, Jeong-Hun Shin 1, Kyung-Soo Kim 1,2, Hyuck Kim 3,✉
PMCID: PMC6170830  PMID: 30603411

Abstract

A systemic treatment of granulocyte-colony stimulating factor (G-CSF) is known to improve healings of damaged tissues. However, recent studies suggested local actions of G-CSF on the healing processes of damaged tissues. We investigated the treatment effect of locally injected G-CSF and compared to that of systemically injected G-CSF in a rat model. A wound was created on the rat dorsum and treated either by local injection or by systemic injection of G-CSF. Wound healing rate, deposition of collagen, and gene expression were evaluated. G-CSF receptor (G-CSFR) protein was detected by Western blotting. The wound healing rate in the local injection group was significantly higher than that in the systemic injection group at days 9 and 15; it was also significantly higher than that in the control group at days 3, 9, and 15. The expression of G-CSFR protein in wound tissues was higher than in normal skin tissues. The local injection of G-CSF is more effective than systemic injection of G-CSF in promoting wound healing, which may implicate the local action of G-CSF treatment in wound healing processes.

Keywords: Granulocyte-colony stimulating factor, Wound healing, Local injection

Footnotes

These authors contributed equally to this work.

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