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. 2016 Feb 2;13(1):13–20. doi: 10.1007/s13770-015-0029-z

Optimization of recombinant human platelet-derived growth factor-BB encapsulated in Poly (lactic-co-glycolic acid) microspheres for applications in wound healing

Yun Ju Bae 1,2, Chi Heung Cho 3, Woo Jong Lee 2, Jeung Soo Huh 4, Jeong Ok Lim 1,5,✉
PMCID: PMC6170986  PMID: 30603380

Abstract

Growth factors play multiple and critical roles in wound repair processes. Platelet-derived growth factor (PDGF) is a potent growth factor that is particularly important in the early inflammatory phase of wound healing. In order to extend the half-life of PDGF, polymeric encapsulation is used. In the current study, Poly (lactic-co-glycolic acid) (PLGA) microspheres containing recombinant human (rh) PDGF-BB were prepared to prolong the effectiveness of this growth factor. PLGA microspheres were optimized using a modified w/o/w-double-emulsion/solvent evaporation method by changing the processing conditions of stirring speed and emulsifier (polyvinyl alcohol) concentration. Microspheres prepared using the optimized method released rhPDGF-BB for up to three weeks. An in vitro migration assay showed a significant decrease in the wound area in cells treated with rhPDGF-BB microspheres compared to control cells. These findings demonstrate the potential of rhPDGF-BB encapsulated in microspheres to enhance wound healing.

Keywords: Recombinant human platelet-derived growth factor-BB, Poly (lactic-co-glycolic acid) microsphere, Wound healing, Human immortalized keratinocyte cell line, Migration

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