There are typographical errors in the fourth and fifth sentences of the Abstract. “IFN-μ” should appear as “IFN-γ.” The correct sentences are: However, to confirm whether a multivalent vaccine can induce an individual epitope-specific CTL, the only viable screening strategies currently available are interferon-gamma (IFN-γ enzyme-linked immunospot (ELISPOT) assays using human peripheral blood mononuclear cells, or expensive human leukocyte antigen (HLA)-expressing mice. In this report, we evaluated the use of our developed murine-20S immunoproteasome (i20S) digestion assay and found that it could predict the results of IFN-γ ELISPOT assays.
There is a typographical error in the Abbreviations. “IFN-μΛ, interferon-gamma” should appear as “IFN-γ, interferon-gamma.”
Reference
- 1.Wada H, Shimizu A, Osada T, Tanaka Y, Fukaya S, Sasaki E (2018) Development of a novel immunoproteasome digestion assay for synthetic long peptide vaccine design. PLoS ONE 13(7): e0199249 10.1371/journal.pone.0199249 [DOI] [PMC free article] [PubMed] [Google Scholar]
