Summary
Centrioles account for centrosomes and cilia formation. Recently, a link between centrosomal components and human developmental disorders has been established. However, the exact mechanisms how centrosome abnormalities influence embryogenesis and cell fate are not understood. PLK4-STIL module represents a key element of centrosome duplication cycle. We analyzed consequences of inactivation of the module for early events of embryogenesis in human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs). We demonstrate that blocking of PLK4 or STIL functions leads to centrosome loss followed by both p53-dependent and -independent defects, including prolonged cell divisions, upregulation of p53, chromosome instability, and, importantly, reduction of pluripotency markers and induction of differentiation. We show that the observed loss of key stem cells properties is connected to alterations in mitotic timing and protein turnover. In sum, our data define a link between centrosome, its regulators, and the control of pluripotency and differentiation in PSCs.
Keywords: centrosome, centriole, stem cell, differentiation, self-renewal, cell cycle, pluripotency, acentrosomal
Graphical Abstract
Highlights
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Blocking of PLK4-STIL module in hESCs/hiPSCs leads to:
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Centrosome loss, prolonged and error-prone mitosis;
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p53-dependent differentiation;
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Reduction of pluripotency linked to altered protein turnover
Recently a link has been established between centrosome and developmental disorders, yet the mechanisms connecting centrosome and cell fate are not understood. Cajanek and colleagues analyzed consequences of centrosome loss using hESCs/hiPSCs. They demonstrated that PLK4/STIL inhibition-mediated centrosome removal leads to loss of key stem cell properties connected to alterations in protein turnover and mitotic timing, which triggers p53-dependent differentiation.
Introduction
The centrosome, an organelle named by Theodor Boveri at the end of the 19th century, has been studied for a long time, but its functions and mechanisms of regulation are still incompletely understood. The centrosome typically acts as a microtubule organizing center (MTOC), taking part in cell division, cell shape organization, and cell motility (Conduit et al., 2015, Khodjakov and Rieder, 2001, Piel et al., 2001). Its core consists of two centrioles, microtubule-based structures with nine-fold radial symmetry, embedded in a protein matrix termed pericentriolar material (Bornens and Gönczy, 2014, Nigg and Stearns, 2011).
The centrosome duplicates once per cell cycle. As a cell divides, each daughter cell inherits one centrosome, so its number in the cells remains stable, similar to DNA content (Bornens and Gönczy, 2014, Nigg and Stearns, 2011). To date, hundreds of centrosomal proteins participating in centrosome biogenesis have been identified (Andersen et al., 2003, Gupta et al., 2015), with PLK4-STIL module having a pivotal role in the orchestration of centriole duplication (Arquint and Nigg, 2016, Bettencourt-Dias et al., 2005, Habedanck et al., 2005, Tang et al., 2011).
Overexpression of essential centrosome regulators, including PLK4, leads to centrosome amplification, whereas their depletion causes loss of centrosomes (Bazzi and Anderson, 2014, Bettencourt-Dias et al., 2005, Habedanck et al., 2005, Leidel et al., 2005, Strnad et al., 2007, Tang et al., 2011). Deregulation of the centrosome duplication cycle is implicated in the etiology of various disorders such as ciliopathies, microcephaly, primordial dwarfism, and cancer (Chavali et al., 2014, Gambarotto and Basto, 2016, Gönczy, 2015, Nigg et al., 2014). However, the consequences of centrosome abnormalities for cell fate have started to be revealed only recently. Inhibition of PLK4 depletes centrioles in various human somatic cell lines, leading to p53-dependent G1 arrest (Lambrus et al., 2015, Wong et al., 2015). In contrast, in vivo study using Drosophila demonstrated that centrosomes are not required for a substantial part of fly embryogenesis (Basto et al., 2006). The requirement for correct embryo development has been further addressed in mice. Mouse embryos without centrosomes die during gestation (Bazzi and Anderson, 2014, Hudson et al., 2001, Izraeli et al., 1999), and amplification of centrosomes after PLK4 overexpression in developing mouse brain leads to microcephaly-like phenotype (Marthiens et al., 2013). That being said, it is becoming clear that cellular outcomes of centrosome abnormalities differ between different models and perhaps even specific cell types (Basto et al., 2008, Levine et al., 2017, Marthiens et al., 2013, Vitre et al., 2015).
Human pluripotent stem cells (PSCs) encompassing both human embryonic stem cells (hESCs) and human induced pluripotent stem cells (hiPSCs) are able to self-renew and to differentiate into all cell types in the human body (Takahashi et al., 2007, Thomson et al., 1998). Pluripotency, governed by a network of transcription factors including OCT-4, SOX-2, and NANOG (Jaenisch and Young, 2008, Kashyap et al., 2009), is tightly connected to cell-cycle regulation (Becker et al., 2006, Pauklin and Vallier, 2013). Importantly, hESCs/hiPSCs hold great promise to model both physiological and pathophysiological aspects of human embryogenesis (Lancaster et al., 2013, Park et al., 2008, Shahbazi et al., 2016). Noteworthy, early passages of human PSCs seem prone to centrosome abnormalities (Brevini et al., 2009, Holubcová et al., 2011). Given these unique properties, we elected to investigate the consequences of halted centrosome duplication cycle in early embryonic events using hESCs and hiPSCs.
Here, we present our analyses of molecular and functional consequences of the inactivation of PLK4-STIL module and centrosome loss for human PSCs. We show that upon centrosome loss, the cells are in principle still able to undergo cell division. Such acentrosomal mitosis is twice as long and leads to mitotic errors and p53 stabilization, which is reflected by gradual loss of self-renewal potential. Interestingly, the observed p53 increase does not lead to significant apoptosis, but to loss of pluripotency and induction of differentiation. Finally, our data demonstrate that the loss of pluripotency regulators after PLK4 inhibition is p53-independent and linked to altered protein turnover.
Results
Blocking of PLK4 or STIL Leads to Centrosome Loss Followed by Decreased Proliferation of Stem Cells
To assess the role of centrosomes in PSCs we used a PLK4 inhibitor, centrinone (Wong et al., 2015). First, we examined the efficacy of centrosome depletion in hESCs following treatment with centrinone. Using immunofluorescence staining for proximal centriolar marker Cep135 (Kleylein-Sohn et al., 2007) and distal centriolar marker CP110 (Chen et al., 2002), we detected the loss of centrosomes in about 40% of hESCs after 2 days (Figures S1A and S1B), and after 3 days the centrosome was depleted in almost 85% of hESCs (Figures 1A and 1B). We were also able to deplete centrosomes in hESCs using PLK4 or STIL short hairpin RNA (shRNA) (Figures S1C and S1D).
It has been recently demonstrated that the loss of centrosomes is detrimental for proliferation of non-transformed human somatic cells, but has little effect on cancer cells (Fong et al., 2016, Lambrus et al., 2015, Meitinger et al., 2016, Mikule et al., 2007, Wong et al., 2015). Given reported similarities in cycle control between embryonic stem cells and cancer cells (Kim et al., 2010), we examined consequences of centrosome depletion for PSC proliferation. Intriguingly, centrinone-treated hESCs/hiPSCs showed impaired proliferation from day 2 and virtually halted their growth past day 5 (Figure 1C). In addition, we also observed a negative effect on proliferation of hESCs following STIL knockdown (Figure 1D). Noteworthy, the negative effect of centrosome loss on proliferation was even more pronounced in the case of hESC-derived neural stem cells (NSCs) (Figure S1E). On the other hand, centrinone treatment showed only a minor effect on proliferation of U2OS cells (Figure S1F), in agreement with the previous report (Wong et al., 2015), even though the efficiency of centrosome depletion was comparable with that of hESCs (Figure S1G).
To corroborate this result, we examined the expression of Ki-67, a marker of proliferating cells. As shown in Figure 1E, centrinone treatment reduced expression levels of Ki-67. In addition, a decrease in the number of Ki-67+ cells was detected in the centrinone condition also by immunofluorescence (Figure S1H, quantified in Figure S1I).
Centrosome Depletion Following PLK4 or STIL Blocking Leads to Prolonged Mitosis and Mitotic Defects
Centrosome loss has been reported to cause various mitotic defects in somatic cell lines (Sir et al., 2013, Wong et al., 2015). Indeed, we noted accumulation of rounded cells in the centrinone-treated cultures and following STIL knockdown (Figure 2A). Furthermore, our subsequent fluorescence-activated cell sorting (FACS) analysis proved that centrinone treatment leads to accumulation of hESCs/hiPSCs in G2/M phase (Figures 2B and 2C).
Next, we analyzed the length of mitosis by live imaging of the reporter H2A-GFP line derived from the same paternal hESC line. As shown in Figure 2D, completion of mitosis between days 2 and 4 took for the treated cells approximately twice as long as controls. In addition, centrinone-treated hESCs showed 1.5-fold prolonged interphase on day 3 compared with control (Figure 2E). All these data indicated an intriguing possibility that centrosome-less hESCs are viable and able to divide, even though for a limited time for the latter. In agreement with this hypothesis we found bipolar mitotic spindles even in acentrosomal cells (Figure S2A). In addition, we quantified the number of cells successfully finishing mitosis in our live imaging experiments. We focused on mitoses past the third day of centrinone treatment, when the majority of treated cells already lacks centrosomes (Figures 1A and 1B). Interestingly, we found 68.1% ± 1.9% of cells able to successfully go through mitosis within the 30-hr period we examined. This observation suggested that acentrosomal mitoses seem possible, but also confirmed our earlier observation (Figure 1C) that proliferation after centrosome loss is inefficient (note 1.5 times longer interphase of centrinone-treated cells; Figure 2E). In addition, we observed cytokinesis failure in approximately 15% of divisions (Figure S2D). To fully prove that acentrosomal hESCs can divide, we performed live imaging experiments with γ-tubulin-GFP hESCs following centrinone treatment (Figure S2G). To conclude, these data argue that centrosome-depleted hESCs are in principle able to successfully finish mitotic division and give rise to two daughter cells, albeit only for a limited time.
In the course of our experiments we noted that nuclei of centrinone-treated cells became bigger and acquired morphology different from control. In agreement with this, FACS analysis detected a modest increase of aneuploid cells after 3 days of centrinone treatment (Figures S2B and S2C). Since it is not possible to distinguish diploid cells residing in G2/M phase from tetraploid cells residing in G1 phase (Figures 2B and 2C) using this approach, it prompted us to quantify the chromosome number. The analysis was done at day 4, when the changes in cell morphology observed during live imaging were most pronounced. Previous work indicated that while centrosome loss during mouse embryogenesis does not lead to notable aneuploidy (Bazzi and Anderson, 2014), somatic cell lines show an increase in chromosomal abnormalities after the centrosome loss (Sir et al., 2013, Wong et al., 2015). Interestingly, our analyses revealed that centrinone treatment of hESCs/hiPSCs led to changes in chromosome number (Figure 2F), arguing that centrosome loss promotes genome instability in PSCs.
Next, to elucidate the survival potential of centrinone-treated cells, we assessed the number of early and late apoptotic cells by annexin V and propidium iodide (PI) staining. We found a modest difference in the number of viable (annexin V/PI negative) cells between centrinone condition and control (Figure 2G). Intriguingly, the proportion of apoptotic cells was notably elevated in hESC-derived NSCs following the centrinone treatment, in contrast to similarly treated cultures of hESCs/hiPSCs (Figure S2E). In addition, we compared the effects of centrinone with those of etoposide, a commonly used DNA-damage-inducing agent. Interestingly, while etoposide triggered a pronounced increase of apoptotic cells in hESC/hiPSC cultures, the percentage of apoptotic cells in centrinone-treated NSCs was similar to the NSC etoposide condition (Figure S2F).
Blocking PLK4 or STIL Promotes Stem Cell Differentiation
Key aspects of PSC biology, the ability to self-renew and to differentiate, are intimately connected to cell-cycle regulation (Becker et al., 2006, Pauklin and Vallier, 2013). Given the phenotypes we found, we examined the impact of centrosome depletion after blocking PLK4 or STIL on those two features.
First, we observed that centrinone-treated cells lost typical stem cell morphology (Figure 3A), suggesting that centrosome loss affects stem cell differentiation. In agreement with this observation, we found a defect in polymerization of microtubules in centrosome-depleted hESCs (Figure S3A). Next, we examined expression of differentiation makers: ectodermal marker PAX-6, endodermal marker GATA-6, and mesodermal marker brachyury. Indeed, mRNA levels of all examined markers were upregulated after centrinone treatment (Figure 3B). Similar effects were confirmed also on the protein level (Figures 3C and S3B). Importantly, our analyses further revealed that protein levels of pluripotency markers OCT-4 and NANOG were decreased in centrinone-treated cells (Figures 3C and S3B). In addition, we detected higher protein levels of p53 in the centrinone conditions, thus confirming and extending previous observations on centrosome loss in somatic cells and mouse embryos (Bazzi and Anderson, 2014, Insolera et al., 2014, Lambrus et al., 2015, Mikule et al., 2007, Wong et al., 2015). Similar effects were also observed after PLK4/STIL shRNA (Figures S3C–S3E). Of note, we did not find a correlation between levels of aneuploidy and brachyury expression (Figures S3F and S3G).
Next, we examined centrinone effects in relation to those of retinoic acid (RA), a commonly used differentiation agent. Intriguingly, upregulation of p53 after centrosome loss was even higher than the effect of RA (Figure S3I). Furthermore, combination of centrinone and RA treatments enhanced PAX-6 protein levels, if compared with either RA treatment alone or untreated condition (Figure S3H).
In the course of our experiments we noted a temporal increase of Ser139 phosphorylated H2AX, a hallmark of DNA-damage response (DDR) (Rogakou et al., 1998) (Figure S3J). Given that DDR typically acts upstream of p53 activation (Brooks and Gu, 2010), we examined a possible role for DDR kinases in the observed increase of p53 levels. However, inhibition of ATM, ATR, and DNA-PK did not prevent the increase (Figure S3K). These data suggest that accumulation of p53 after PLK4 or STIL blocking-induced centrosome loss in hESCs is independent of DDR signaling.
Given this result, we considered alternative routes of p53 upregulation. First, we found that knockdown of dicer-1, a key regulator of microRNA biogenesis, does not prevent p53 upregulation (Figure S3L), indicating that microRNA machinery is not involved in the p53 activation. Next, using cycloheximide (CHX) to block translation, we found that p53 protein moiety is stabilized following centrinone treatment (Figure 3D). Furthermore, we examined effect of prolonged mitosis on p53 stabilization. To mimic the effect of centrosome depletion on the mitotic length, we treated hESCs for 2 hr (Figure S3M) or hESCs/hiPSCs for 6 hr by nocodazole to arrest them in mitosis, isolated mitotic cells by shake-off, then released them by washout and analyzed them 2 days following release. Remarkably, we found upregulated p53, brachyury, and GATA-6 in cells that experienced temporal mitotic arrest (Figure 3E). Of note, we occasionally observed an increase also in PAX-6 levels (Figure 3E), perhaps reflecting requirement of concomitant downregulation of OCT4/NANOG for the efficient PAX-6 induction (see Figure S4B). Importantly, cells treated with nocodazole, but not passing through mitotic arrest (“Noco-leftover”), showed expression of examined markers at levels comparable with that of control. Thus, the data demonstrate that prolonged mitosis is sufficient to trigger p53 upregulation and cell differentiation.
Differentiation Induced by Blocking of PLK4 or STIL Is p53 Dependent
Previous studies linked p53 to induction of differentiation in PSCs (Jain et al., 2012, Lin et al., 2005, Qin et al., 2007, Zhang et al., 2014). On the other hand, there are contradictory reports about the expression and/or activity of p53 in hESCs/hiPSCs (Aladjem et al., 1998, Maimets et al., 2008, Momcilovic et al., 2009, Qin et al., 2007, Wang et al., 2016, Zhang et al., 2014). To examine the role of p53 in the differentiation observed in our experiments, we first downregulated it using small interfering RNA (siRNA) (Figure 4A). We found that the knockdown of p53 has no apparent effect on mitotic cell accumulation following centrinone treatment (Figure 4B). Importantly, downregulation of p53 expression by either siRNA in hESC cultures or CRISPR/Cas9 system (p53 low hESCs/hiPSCs) only partially rescued the proliferation defect seen after centrosome loss (Figure 4C). Thus, these results not only confirmed and extended previous observations from somatic cells (Fong et al., 2016, Lambrus et al., 2016, Meitinger et al., 2016, Wong et al., 2015), but also suggested the interesting possibility that some of the phenotypes caused by centrinone treatment in hESCs/hiPSCs might be p53 independent. To this end, we examined the requirement of p53 for centrosome loss-induced differentiation of PSCs. We found that depletion of p53 prevented the upregulation of T and PAX6 mRNA in hESCs (Figure 4D). Furthermore, centrinone treatment led to higher protein levels of brachyury in control condition, but not in cells with depleted p53 by siRNA (Figure 4E). Intriguingly, expression of OCT-4 was not rescued by p53 depletion. To corroborate this finding, we performed a similar experiment using p53 low hESCs/hiPSCs (Figures 4F and 4G). As expected, we found full dependency of the induction of brachyury expression on the presence of p53. Importantly, however, OCT-4 protein was downregulated even in conditions without detectable levels of p53 (Figure 4F). Thus, these data demonstrated that the induction of differentiation markers after centrinone-mediated centrosome loss requires p53, while the loss of pluripotency markers is p53 independent.
Loss of Pluripotency after PLK4 Inhibition and Centrosome Depletion Is Linked to Altered Protein Turnover
To elucidate the mechanism responsible for the downregulation of regulators of pluripotency after centrinone treatment, we first analyzed its effects on POU5F1 and NANOG mRNA levels. Surprisingly, we found no difference in either POU5F1 or NANOG mRNA levels (Figure S4A). This result indicated post-transcriptional regulation and prompted us to examine protein stability of OCT-4/NANOG. Interestingly, we found increased turnover of OCT-4/NANOG in the centrinone-treated hESCs (Figure 5A). Given that levels of p53 and β-catenin, included as controls in our experiments, did not follow the same trend as OCT-4/NANOG, these data confirmed the specificity of the effect. Next, we examined whether prolonged mitosis, induced by nocodazole, is sufficient to alter turnover of OCT-4/NANOG. However, in contrast to the effects seen on upregulation of p53 and other differentiation markers (Figure 3E), prolonged mitosis did not show an effect on OCT-4/NANOG turnover (Figure S4B).
To identify the degradation pathway responsible for turnover of OCT-4/NANOG, we used MG132 and chloroquine, inhibitors of proteasome and lysosome, respectively. While the treatment with chloroquine showed no effect (Figure S4C), addition of MG132 to CHX-treated cells showed a rescue effect on the drop in protein levels of OCT-4 and NANOG, indicating that these transcription factors are subjected to proteasomal degradation in both control and centrinone-treated cells (Figures 5B and S4C). As PLK4 was shown to regulate protein turnover of SAS-6 (Puklowski et al., 2011), we examined its possible direct role in turnover of OCT-4. First we tested that washing out centrinone is sufficient for reactivation of PLK4 in hESCs (Figures S4D–S4G), in agreement with previous reports on somatic cells (Lambrus et al., 2015, Wong et al., 2015). Importantly, however, restoring PLK4 activity in centrosome-depleted cells did not show any rescue effect on OCT-4 (Figure S4H).
We observed that the centrinone-treated cells were somewhat more sensitive, as they showed increased levels of cleaved poly(ADP ribose) polymerase (PARP) and cleaved caspase-3 (Figure 5B). Importantly, this phenomenon was triggered by the CHX treatment, as hESCs/hiPSCs treated only with centrinone showed no upregulation of apoptotic markers. Given these results, we hypothesized that the observed stress response of centrinone-treated cells to CHX treatment might reflect the accelerated loss of specific proteins in these cells. To this end, we aimed to test the causality between the observed priming of centrinone-treated stem cells to enter the apoptotic pathway and altered protein turnover. However, MG132 treatment, in agreement with its typical use in anti-tumor therapy (Goldberg, 2012), led to an increase of p53 and cell-death markers in hESCs/hiPSCs, indicated by the appearance of cleaved PARP and caspase-3 (Figure 5B). To bypass these undesired effects of MG132, we turned to p53 low hESCs. Interestingly, co-treatment of centrosome-depleted and CHX-treated hESCs with MG132 showed full rescue of the drop in OCT-4/NANOG levels (Figure 5C). Moreover, CHX treatment caused modest effects on cleaved PARP and caspase-3, respectively. Interestingly, those were fully rescued by the MG132 treatment. To corroborate our hypothesis, we tested the ability of MG132 to rescue the activation of the apoptotic pathway after centrosome loss in p53 low hESCs/hiPSCs. As expected, centrinone treatment alone led to only a negligible fraction of early apoptotic cells, while co-treatment with CHX increased this fraction (Figure 5D). Remarkably, addition of MG132 was able to notably decrease the proportion of early apoptotic cells, specifically in centrinone + CHX conditions. Together, these results demonstrated that p53 is not necessary for the increased protein turnover of OCT-4/NANOG in centrinone-treated cells, and that block of excessive protein degradation in centrosome-depleted cells is sufficient to lower the stress response and priming of these cells to apoptosis.
Discussion
Centrosome abnormalities are related to detrimental developmental defects. Here we have explored the link between the loss of centrosome and the cell fate in hESCs/hiPSCs and have shown that depletion/inhibition of PLK4 or depletion of STIL lead to centrosome depletion, and in turn to prolonged mitosis, which consequently leads to p53 upregulation and subsequent differentiation. We further established the PLK4 inhibition-mediated and/or centrosome depletion-mediated loss of pluripotency independent of p53 and linked to altered protein turnover.
Our data indicate that a large portion of acentrosomal hESCs/hiPSCs is able to divide. Importantly, our experiments further showed that centrosome loss promotes aneuploidy in hESCs/hiPSCs, a phenomenon usually seen in somatic/cancer cell lines but not in vivo in mouse embryos (Bazzi and Anderson, 2014, Insolera et al., 2014, Sir et al., 2013, Wong et al., 2015). It remains to be determined whether this “mouse embryo versus human cells” difference reflects specific aspects of cell culture or is linked to an acentrosomal period of early embryogenesis in mouse (Szollosi et al., 1972). Either way, our data establish that the loss of centrosome in hESCs/hiPSCs contributes to genome instability.
The proliferation rate of centrosome-less hESCs/hiPSCs was impaired, consistent with reports on human somatic cells (Lambrus et al., 2015, Wong et al., 2015) or mouse embryo (Bazzi and Anderson, 2014). Studies on somatic cells also proposed that the proliferation defect seen after centrosome loss is fully dependent on p53 (Lambrus et al., 2015, Wong et al., 2015). Interestingly, however, depletion of p53 by RNAi or CRISPR/Cas9 showed only moderate rescue of proliferation defect after centrosome loss, while it completely prevented the induction of differentiation markers. Even though we cannot formally exclude the effects of different experimental designs, we conclude that self-renewal defect in centrosome-depleted hESCs/hiPSCs following PLK4 or STIL blocking is dependent on p53 only partially.
Previous studies linked p53 to induction of differentiation in PSCs (Jain et al., 2012, Lin et al., 2005, Qin et al., 2007, Zhang et al., 2014). However, the ability of activated p53 to directly repress transcription of any gene has been recently challenged (Allen et al., 2014), and the possible role of p53 in direct repression of pluripotency factors is rather controversial (Aladjem et al., 1998, Maimets et al., 2008, Momcilovic et al., 2009, Qin et al., 2007, Wang et al., 2016, Zhang et al., 2014). In addition, a recent report by Gogendeau et al. (2015) postulated that aneuploidy-induced differentiation of NSCs in Drosophila is largely p53-independent. With all that said, our data clearly point out the requirement of p53 for the induction of differentiation markers after inactivation of PLK4-STIL module and centrosome loss. Importantly, however, loss of pluripotency markers upon centrinone treatment was not rescued by p53 depletion, suggesting that the loss of pluripotency is p53-independent. This is in agreement with recent reports on Nanog expression during differentiation of p53 null mouse ESCs (Shigeta et al., 2013, Wang et al., 2016) and work on p53-deficient mice reporting no developmental defects (Donehower et al., 1992). Furthermore, this model predicts that the control of pluripotency and the induction of differentiation following centrosome depletion are interconnected but autonomously regulated phenomena, with p53 playing an instructive role in the latter. That being said, we speculate that the involvement of p53 in centrosome loss-driven differentiation explains why we and others (Amps et al., 2011, Ben-David et al., 2014, Taapken et al., 2011, Zhang et al., 2016) find no evidence for aneuploidy being the main driving force of upregulation of differentiation markers, as reported for p53-independent differentiation of Drosophila NSCs (Gogendeau et al., 2015).
Activation of p53 is a typical response to DDR. However, observations from us (this study) and others (Lambrus et al., 2015, Wong et al., 2015) demonstrate that the activation of p53 after centrosome loss is DDR-independent. Recent studies provided hints about events upstream of p53 activation in centrosome-less somatic cells by pointing out the requirement for TP53BP1 and USP28 (Fong et al., 2016, Lambrus et al., 2016, Meitinger et al., 2016). It is possible that this module operates also in PSCs. However, the exact nature of the putative stress signal activating p53 remains elusive. Our data indicate that the prolonged mitosis, one of the earliest consequences of centrosome loss, is sufficient to trigger p53 upregulation and differentiation in hESCs/hiPSCs. This finding has two pertinent consequences. First, any experiments with PSCs involving mitotic drugs need to be interpreted with caution, as such treatment may directly interfere with their undifferentiated status. Furthermore, it raises a question about the role of prolonged mitoses following centrosome loss. As already mentioned, prolonged mitosis is sufficient to trigger differentiation via induction of p53. However, removal of p53 is not able to sustain self-renewal of centrosome-depleted hESCs/hiPSCs. In addition, we were not able to mimic the effect of PLK4 or STIL blocking on downregulation of OCT-4/NANOG by the prolongation of mitosis. Thus, it seems plausible that defects observed in hESCs/hiPSCs following inactivation of the PLK4-STIL module reflect impairment of both mitotic and non-mitotic function of the centrosome.
We found that the decrease of OCT-4/NANOG after centrinone treatment is caused by faster turnover of these proteins, independently of p53. The fact that active PLK4 is not able to rescue downregulation of OCT-4 in centrosome-depleted cells supports the conclusion that the enhanced turnover is a consequence of centrosome loss rather than inhibition of PLK4. We speculate that altered proteasomal activity, in combination with elevated stress response, might contribute to this phenomenon (Bryja et al., 2017, Gerdes et al., 2007, Vora and Phillips, 2015). In addition, the increased degradation of NANOG/OCT-4 could be a consequence of more complex metabolic changes. This prediction is supported by the rescue effect of MG132 treatment on induction of apoptosis in our experiments, possibly due to prevention of a loss of pro-survival factors upon block of protein synthesis (Portt et al., 2011).
In sum, our study defines a novel role for PLK4-STIL module and the centrosome in the regulation of key stem cell properties. It identifies both p53-dependent and -independent consequences of inactivation of the module in PSCs and connects them to alterations in mitotic timing and protein metabolism. Future studies on the links between centrosome, proteasome regulation, and apoptotic response could contribute to a better understanding of the pathology of centrosome-related diseases.
Experimental Procedures
Cell Lines
hESCs (line CCTL14, https://hpscreg.eu/cell-line/MUNIe007-A), hESCs 2 (CCTL12, https://hpscreg.eu/cell-line/MUNIe005-A; “hESCs #2”) (Adewumi et al., 2007, Bohaciakova et al., 2017), and hiPSCs (derived as described previously; Barta et al., 2016) were cultured, treated, and analyzed as described in detail in Supplemental Experimental Procedures.
Statistical Analysis
All statistical analyses were done using Student's t test and graphically visualized in GraphPad Prism Software v. 6.0 (GraphPad Software, La Jolla, CA; www.graphpad.com). All data are presented as mean ± SEM from three independent experiments, unless otherwise stated, and p values <005 were considered significant (∗p < 0.05, ∗∗p < 0.005, ∗∗∗p < 0.001, ∗∗∗∗p < 0.0001 in figures).
Western Blots
Detailed protocol is described in Supplemental Experimental Procedures; western blot quantifications and all independent repeats are shown in Supplemental Western Blot Data.
Additional experimental procedures are provided in Supplemental Information. A list of used antibodies, primers, and shRNA constructs is provided in Table S1.
Authors Contributions
T.R. designed and performed experiments, and analyzed and interpreted data; D.B. performed experiments, and analyzed and interpreted data; M.E. and V.P. performed experiments and analyzed data; T.B. and A.H. provided critical reagents; L.C. conceived and supervised the study, analyzed and interpreted data, and together with T.R. wrote the manuscript. All authors approved the final version of the manuscript.
Acknowledgments
We thank Erich Nigg, Andrew Shiau, Borivoj Vojtesek, and Anthony Hyman for sharing reagents, Lumir Krejci for access to DV Elite, Pavlina Janovska for help with FACS analyses, Klara Koudelkova and Karolina Hanzakova for assistance, Vitezslav Bryja, Zdenek Andrysik, and members of the L.C. lab for critical comments and suggestions. We acknowledge the core facility CELLIM of CEITEC, supported by the MEYS CR (LM2015062 Czech-BioImaging). This work was supported by grants from the SoMoPro II Program (project 4SGA8574), cofinanced by European Union and the South-Moravian Region; Czech Science Foundation (16-03269Y); Swiss National Science Foundation (IZ11Z0_166533); follow-up research fund from Federation of Biochemical and Biophysical Societies (FEBS); Grant Agency of Masaryk University (GAMU grant category E), and funds from Medical Faculty MU to Junior researcher (ROZV/24/LF2016) to L.C. D.B. and T.B. were supported by the Czech Science Foundation (15-18316Y, 16-24004Y, and 18-25429Y), and funds from Medical Faculty MU to Junior researcher (ROZV/25/LF/2017, ROZV/24/LF/2018 and MUNI/G/1131/2017). A.H. was supported by Czech Science Foundation (15-11707S) and by the project no. LQ1605 (MYES CR, NPU II). The content of this publication reflects only the authors' views, and the European Union is not liable for any use that may be made of the information contained therein.
Published: September 6, 2018
Footnotes
Supplemental Information includes Supplemental Experimental Procedures, four figures, and one table and can be found with this article online at https://doi.org/10.1016/j.stemcr.2018.08.008.
Supplemental Information
References
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