FIG 4.

Pairwise nucleotide comparison of mcr-1-positive multidrug resistance plasmids pSA26-MCR1 and pMS8345. (A) A comparison of mcr-1-positive plasmids from Qatar and Saudi Arabia reveal them to have highly similar backbones. However, both plasmids differ considerably in their resistance gene complement, clearly visible as a large, highly variable MDR region characterized by numerous insertions, deletions, inversions, and rearrangements. The MDR island is represented by a red rectangle and the mcr-1 region by a green rectangle. (B) Comparison of the ISApl1-mcr-1 mobile elements from pMS8345A and pSA26-MCR1. ISApl1 mobile elements are represent by red rectangles. Black arrows indicate the orientation of the insertion sequence (IS) elements, and protein-coding regions are represented by the colored arrows.