Table 1. Effect of carbon sources, nitrogen sources, and cultivation conditions on exopolysaccharide production.

The different carbon sources, organic and inorganic nitrogen and media composition, cultivation conditions were investigated.

Medium sources	EPS yield (g/L)
	EPS-YO175	EPS-OF101
Carbon sources (20 g/L)
Glucose	0.94 ± 0.10	0.62 ± 0.44
Sucrose	1.59 ± 0.10	1.07 ± 0.26
Lactose	1.48 ± 0.14	1.03 ± 0.17
Galactose	0.87 ± 0.26	0.41 ± 0.26
Sucrose concentrations (g/L)
10	1.13 ± 0.20	0.90 ± 0.62
20	1.64 ± 0.44	1.05 ± 0.44
30	1.52 ± 0.26	0.71 ± 0.56
40	1.45 ± 0.75	0.62 ± 0.17
50	1.10 ± 0.00	0.36 ± 0.10
Organic nitrogen (25 g/L)
Yeast extract	1.63 ± 0.07	1.01 ± 0.11
Beef extract	1.15 ± 0.22	0.82 ± 0.10
Tryptone	1.48 ± 0.31	0.83 ± 0.24
Peptone	1.61 ± 0.01	0.77 ± 0.20
Inorganic nitrogen (2 g/L)
Ammonium sulphate	1.56 ± 0.10	1.00 ± 0.00
Ammonium nitrate	1.51 ± 0.00	0.80 ± 0.00
Ammonium chloride	1.48 ± 0.21	0.71 ± 0.10
Tri-ammonium citrate	1.37 ± 0.32	0.75 ± 0.12
Sodium nitrite	1.30 ± 0.10	0.68 ± 0.26
Potassium nitrate	1.15 ± 0.24	0.72 ± 0.18
Temperature (°C)
20	0.53 ± 0.20	0.20 ± 0.01
25	0.77 ± 0.01	0.31 ± 0.08
30	1.56 ± 0.10	1.03 ± 0.11
37	1.50 ± 0.18	0.80 ± 0.00
45	0.46 ± 0.24	0.10 ± 0.10
Cultivation time (h)
12	0.30 ± 0.06	0.10 ± 0.04
24	1.70 ± 0.08	0.76 ± 0.10
36	1.66 ± 0.24	1.00 ± 0.12
48	1.68 ± 0.20	1.08 ± 0 22
60	1.50 ± 0.10	1.02 ± 0.24
72	1.38 ± 0.07	0.72 ± 0.11
84	1.17 ± 0.04	0.66 ± 0.22
96	1.00 ± 0.30	0.60 ± 0.32
pH
6.0	1.51 ± 0.11	0.65 ± 0.01
6.5	1.60 ± 0.00	1.01 ± 0.01
7.0	1.72 ± 0.24	1.10 ± 0.02
8.0	1.50 ± 0.33	0.82 ± 0.07

Notes.

Values are means of three independent experiments (mean ± SD).