Fig. 5.

Mapping of cortical cyto-architectonics in 3D within the volume of typical MRI voxel. (a) Reconstruction of a 3D image of cortex subsample (1 A) with neuronal cell bodies stained for HuC/D. Y-axis corresponds to the direction from white matter to pial surface and z-axis corresponds to the imaging depth direction. (b) Results of classification of neuronal cell body morphometry to pyramidal (blue) and non-pyramidal (red) neurons. Cortical layers III and IV are clearly identified throughout the entire imaged volume of 2.6 mm indicating a consistent imaging quality throughout the sample. (c) Projection of classified volume over the z-direction. Layers III and IV can be clearly recognized.