Table 2.
Primary antibodies used for immunohistochemical staining of cleared post mortem human brain tissue.
| Cell Types/Compartments | Detected Protein | Antibodies | Dilution | Source |
|---|---|---|---|---|
| Myelin and fibers | ||||
| Myelinated fibers | Myelin basic protein (MBP) | Rat anti-MBP | 1:400 | Abcam |
| Microtubules | ß-III-Tubulin | Rabbit anti-ß-III-Tubulin | 1:1 000 | Sigma |
| Cellular markers | ||||
| Neurons | Human neuronal Protein C/D (HuC/D) | Mouse anti-HuC/D | 1:400 | Invitrogen |
| Fast spiking interneurons | Parvalbumin | Rabbit anti-PV25 | 1:500 | Swant |
| Glia markers | ||||
| Microglia | Ionized calcium binding adaptor1 (Iba-1) | Rabbit anti-Iba1 | 1:500 | Wako |
| Astroglia | Glial fibrillary acidic protein (GFAP) | Rabbit anti-GFAP | 1:1 500 | Dako |
| Astroglia | Glial fibrillary acidic protein | Mouse anti-GFAP | 1:1 000 | Sigma |
| Astroglia | Glial fibrillary acidic protein | Guinea-Pig anti-GFAP | 1:500 | Synaptic Systems |
| Vessels | ||||
| Vessel walls | α-D- and N-acetyl-Galactosamine residues | helix aspersa agglutinin, biotinylated | 1:100 | Sigma |