FIG 8.

BadM DNA binding to the badDEFG promoter is reduced in the presence of acetylated 2-aminobenzoate. (A) Binding of BadM (72 nM) to the badDEFGAB promoter (12 nM) was analyzed by electrophoretic mobility shift assays using 6-FAM 5′-labeled probes. (A) Probe containing the intergenic region of badCD (212 bp, 0.303 pmol) was incubated with BadM (1.8 pmol) or without BadM. The addition of 2-aminobenzoate or 2-acetamidobenzoate is indicated above the respective lanes. (B) Binding of BadM to the badCD intergenic region as in panel A, but with different concentrations of 2-aminobenzoate or 2-acetamidobenzoate.