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. 2018 Oct 16;9(5):e01954-18. doi: 10.1128/mBio.01954-18

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Copyright © 2018 Brown et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 5 — Phylogenetic tree of EV-D68 isolates based on VP1 sequences. VP1 nucleotide sequences of >900 nt were retrieved from the ViPR site (https://www.viprbrc.org/brc/home.spg?decorator=picorna_entero) on 24 July 2017. Sequences were aligned using the MUSCLE algorithm and sequences showing poor alignments were removed. A phylogenetic tree was computed using RaXML (bootstrap replicates of 100) and then visualized using Archaeopteryx.js via the ViPR site. Clade classifications are based on bootstrap values of 100%. AFM-associated isolates are marked with a green arrow. EV-D68 isolates used in this study are labeled with either a pink (growth in SH-SY5Y cells) or orange (no growth in SH-SY5Y cells) arrow. Isolates that are paralytogenic in mice (56) are labeled with a blue arrow.