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. Author manuscript; available in PMC: 2018 Oct 17.
Published in final edited form as: Pancreas. 2017 Oct;46(9):1202–1207. doi: 10.1097/MPA.0000000000000913

FIGURE 3. Paracrine TGF-β from ductal cells promotes ADM of primary human pancreatic acinar cells in vitro.

FIGURE 3.

A, Five days after culture, a significant proportion of CLA−CD133− cells (blue, acinar cells) gained CD133 expression to become CLA−CD133+ cells (red, AD cells). The TGF-β binding protein BGeRII (depletion of secreted TGF-βs) treatment inhibited CD133 up-regulation in acinar cells (n = 3). B, Sorted acinar cells did not gain CD133 expression when cultured alone in the 2D system. c) Both TGF-β1 and conditioned media from cultured ductal cells induced the conversion of acinar cells to AD cells. BGeRII treatment abolished the inductive effects of condition media (n = 3). C, Five days after culture, TGF-β1 mRNA was up-regulated in both acinar and ductal cells. However, the expression of TGF-β1 in acinar cells was significantly lower than that in ductal cells (n = 3). Data represent mean (SD) from three independent experiments, ***P < 0.001.