Arrhythmogenic Mechanisms in Heart Failure: Linking β-Adrenergic Stimulation, Stretch, and Calcium

Abstract

Heart failure (HF) is associated with elevated sympathetic tone and mechanical load. Both systems activate signaling transduction pathways that increase cardiac output, but eventually become part of the disease process itself leading to further worsening of cardiac function. These alterations can adversely contribute to electrical instability, at least in part due to the modulation of Ca²⁺ handling at the level of the single cardiac myocyte. The major aim of this review is to provide a definitive overview of the links and cross talk between β-adrenergic stimulation, mechanical load, and arrhythmogenesis in the setting of HF. We will initially review the role of Ca²⁺ in the induction of both early and delayed afterdepolarizations, the role that β-adrenergic stimulation plays in the initiation of these and how the propensity for these may be altered in HF. We will then go onto reviewing the current data with regards to the link between mechanical load and afterdepolarizations, the associated mechano-sensitivity of the ryanodine receptor and other stretch activated channels that may be associated with HF-associated arrhythmias. Furthermore, we will discuss how alterations in local Ca²⁺ microdomains during the remodeling process associated the HF may contribute to the increased disposition for β-adrenergic or stretch induced arrhythmogenic triggers. Finally, the potential mechanisms linking β-adrenergic stimulation and mechanical stretch will be clarified, with the aim of finding common modalities of arrhythmogenesis that could be targeted by novel therapeutic agents in the setting of HF.

Introduction

Heart failure is a complex clinical syndrome with many contributory factors including ischemia, congenital heart disease, and pulmonary hypertension. HF can be defined as HF with preserved ejection fraction (HFpEF), when diastolic dysfunction plays a major role, or HF with reduced ejection fraction (HFrEF). HF with reduced ejection fraction has been associated with elevated sympathetic tone and mechanical load (Lohse et al., 2003). Both systems activate signaling transduction pathways that increase cardiac output, but adversely contribute to electrical instability, at least partially via modulation of Ca²⁺ handling.

The first documentation of alterations in the sympathetic signaling in chronic HF was when a decrease in concentrations of the sympathetic nervous neurotransmitter, norepinephrine, was shown in the failing human heart (Chidsey et al., 1963). Since that time, there has been accumulating evidence that the sympathetic nervous system plays a considerable role in HF (Port and Bristow, 2001) and this is highlighted by the continued use of β-receptor blockers as a favorable pharmacological treatment of HF (Waagstein et al., 1993; Ponikowski et al., 2016).

The hyperadrenergic state is in large part caused by an imbalance of autonomic reflex responses to early alterations in cardiac and peripheral hemodynamics (Toschi-Dias et al., 2017). In HF, vagal control by the baroreceptor reflex is reduced (Eckberg et al., 1971), while sympatho-excitatory reflexes are augmented, including the cardiac sympathetic afferent reflex (Wang and Zucker, 1996). The cardiac-specific reflex originates in the ventricle and is activated by elevated filling pressures (Malliani et al., 1973; Wang and Zucker, 1996), creating a positive feedback loop as its activation causes excessive sympathetic outflow to the heart and arteries (Chen et al., 2015). In turn, the heart readapts its systolic and diastolic force to the adrenergic-mediated increases in hemodynamic load via intrinsic autoregulatory mechanisms (Neves et al., 2015). Thus, adrenergic and hemodynamic regulatory systems tightly interact via a complex interplay of feedback mechanisms at the local and systemic level that are initially compensatory, but ultimately pathological.

Arrhythmias are a major cause of mortality in HF patients, and sudden cardiac death has previously been linked with a higher NYHA class (Saxon et al., 2006; Santangeli et al., 2017). Furthermore, in a recent study, ventricular arrhythmias were seen in up to 45% of patients who had received a LVAD (Garan et al., 2013). Although over the last decades remarkable advances have been made in terms of our understanding of risk factors and the efficacy of device therapy the underlying mechanisms responsible for arrhythmia induction and sudden cardiac death in this population remain elusive, and this is largely down to the complexity of the disease.

In this review, we will focus on the roles that altered sympathetic stimulation as well as mechanics may have on arrhythmogenic phenotype in patients with HF with reduced ejection fraction, concentrating on alterations of Ca²⁺ dynamics, β-adrenergic stimulation and stretch at the level of the single cardiac myocyte. It is hoped that information gained in this field will ultimately lead to novel strategies that could improve our therapeutic arsenal against HF.

Basic Principles of Calcium-Dependent Arrhythmogenesis-Afterdepolarizations

Before discussing arrhythmogenic mechanisms in HF we need to understand the basic mechanisms of arrhythmogenesis and the link to Ca²⁺.

Afterdepolarizations are thought to be one of the major mechanisms driving arrhythmogenesis in multiple patho-physiologies and we will concentrate on these in this review (Figure 1). These oscillations in the membrane potential can lead to either triggered activity and/or functional block which may encourage re-entry circuits (Wit and Rosen, 1983). These phenomena can be detected at multiple levels, ranging from the single cardiac myocyte to the tissue and can even be observed in the intact heart when monophasic APs are recorded (Priori et al., 1990). They are defined as depolarizations of the cardiac AP that can occur in phases 2, 3, or 4 of the AP (Cranefield, 1977). When they occur in phase 4 of the AP they are called DADs whereas if they occur earlier on the AP then they are termed EADs.

FIGURE 1.

Stylized examples of afterdepolarizations occurring in the single canine myocyte. Figure shows both membrane potentials and contraction for each situation. (Left) DAD (^∗) induced by the β-adrenergic agonist isoproterenol (ISO); (Middle) illustrates an EAD (#) induced by augmentation of the late sodium current, using ATX-II; (Right) illustrates that under certain conditions both types of afterdepolarizations can be seen in the same action potential. In this particular example, blockade of the potassium current, I_Ks, together with β-adrenergic stimulation are the proarrhythmic treatment and it can be seen that an early aftercontraction initiates prior to the upstroke of the EAD.

There is now a general consensus that DADs are a result of a I_TI activated by intracellular Ca²⁺ (Marban et al., 1986). This I_TI was originally described by Lederer and Tsien (1976) as a result of digitalis-induced arrhythmias and was later shown to be mainly due to activation of the electrogenic Na⁺-Ca²⁺ exchanger (NCX), while the Ca²⁺-activated Cl^- current (ICl_Ca) appears to contribute in some species (Fedida et al., 1987; Zygmunt et al., 1998). Interestingly a study from Verkerk et al. (2001) showed that I_TI in failing human cardiac myocytes was as a result of NCX alone. In addition to the potential of DADs to induce arrhythmias via triggered activity or functional block, recent work has also highlighted the potential of DADs to increase beat-to-beat variability of repolarization (BVR) which may also contribute to the arrhythmogenic nature of these afterdepolarizations (Johnson et al., 2013). The Ca²⁺ that activates these arrhythmogenic currents originate from the SR and is released via diastolic release events (Venetucci et al., 2008). For these reasons, when the SR is overloaded with Ca²⁺, for example during intense β-adrenergic stimulation, the chance of spontaneous Ca²⁺ release is increased as is the likelihood for DADs (Yamada and Corr, 1992).

Mechanisms underlying EADs are much less clear cut however, and remain a topic of much debate. Early evidence suggested EADs were caused as a result of reactivation of I_CaL due to the prolonged plateau phase of the AP (January and Riddle, 1989; Zeng and Rudy, 1995). However, there is other experimental evidence that suggests that EADs may also be caused as a result of I_TI activation by intracellular Ca^2+, especially under conditions of Ca²⁺ overload (Priori et al., 1990; Volders et al., 1997, 2000). Either way, it appears that EADs are modulated by systolic release of Ca²⁺ during the AP and are regulated by feedback on Ca²⁺ sensitive ion currents.

The link of afterdepolarizations at the single myocyte level to arrhythmogenesis at the whole heart level is extremely complex and incompletely understood. In the intact heart, myocytes are electrically coupled to each other, meaning that the membrane voltage is governed by not just one cell but multiple cells. Furthermore, the contribution of one afterdepolarization occurring in one cell (the source) will likely be negated by the neighboring cells not experiencing afterdepolarizations (the sink). Elegant work from the laboratory of James Weiss has investigated this phenomenon and has shown that chaotic EADs are able to synchronize globally when the tissue is smaller than a critical size. However, when the tissue is large enough, complete synchronization of EADs can no longer occur and this results in regions of partial synchronization that shift in time and space, that can act as foci for arrhythmia (Sato et al., 2009). Furthermore, work from the same group also estimated that the number of local myocyte DADs needed to be synchronized to induce a premature beat would be very large, however, this could be reduced structural and electrical remodeling (Xie et al., 2010). Finally, a recent study from the Bers’ lab has also highlighted that in HF there is a much higher density of ‘Ca²⁺ asynchronous’ myocytes that are poorly coupled to the surrounding myocardium. These poorly coupled myocytes may also contribute to initiating triggered activity (Lang et al., 2017).

Over recent years it has become apparent that Ca²⁺ control of repolarization, and therefore also of arrhythmogenesis is more of a local rather than a global phenomenon. Both functional and structural microdomains dictate local Ca²⁺ concentrations, gradients and effector proteins. As these domains appear to be affected in HF, especially when we consider β-adrenergic stimulation and stretch we must also consider how this local control occurs.

Local Control of Ca²⁺ Release at the Dyad

In cardiac myocytes, Ca²⁺ is centrally involved in many processes including excitability, contraction, and regulation of gene expression. Such diversity of functional roles postulates the existence of dedicated microdomains in which Ca²⁺ signals are generated independently of cytosolic Ca²⁺ concentrations and sensed by macromolecular signaling components localized to these microdomains. Besides a functional component, Ca²⁺ microdomains are often physically delimited by specialized membrane structures and subcellular compartments. Specialized structures include dyadic junctions between transverse membrane invaginations (T-tubules) and SR, sarcolemmal domains outside dyads such as lipid rafts and caveolae, and intracellular structures such as tethered junctions between SR and mitochondria. Interestingly, compartmentalization of proteins that generate or regulate microdomain Ca²⁺ signaling is dynamic, often as a cause or consequence of disease. For example, the LTCC, or nNOS coupled to RyR, can translocate from the dyad to the sarcolemma which alters their function, presumably by coupling to different signaling complexes (Sanchez-Alonso et al., 2016; Carnicer et al., 2017).

Structural Organization

In the dyad, RyR in the SR juxtapose LTCC along T-tubules at close distances (∼12 nm) (Forbes and Sperelakis, 1983). Individual dyads control the process of Ca²⁺ induced Ca²⁺ release, or CICR. The elementary event is a Ca²⁺ spark released from the SR by the opening of RyR in a single Ca²⁺ release unit (Cheng and Lederer, 2008). When an LTCC opens during an AP, the local Ca²⁺ concentration in the dyad raises much more than cytosolic Ca²⁺, from a diastolic level of 100 nM to more than 10 μM, sufficiently to activate RyR (Cannell and Kong, 2012). Not all RyR are localized at dyads; non-coupled RyR are activated through propagated Ca²⁺ release with a delay. Therefore, a large heterogeneity of the tubular system (e.g., due to T-tubule loss in HF) causes dyssynchrony of subcellular Ca²⁺ release during systole (Heinzel et al., 2011). During diastole, few spontaneous Ca²⁺ sparks occur due to the relatively low sensitivity of RyR to resting Ca²⁺ levels. A spontaneous release event is spatially confined, but when the Ca²⁺ sensitivity or the RyR increases through phosphorylation or oxidation, or when SR Ca²⁺ load is high, more spontaneous Ca²⁺ sparks summate in time and space into propagating Ca²⁺ waves.

The structural design of the dyad also provides an optimal setting for feedback mechanisms of SR Ca²⁺ release on Ca²⁺-regulated membrane currents. Negative feedback through Ca²⁺ release-dependent inactivation of LTCC serves as a mechanism to limit Ca²⁺ influx during the initial phase of the AP (Sham, 1997). As release-dependent inactivation is immediate, following the fast rise and decline of local Ca²⁺ near dyads, some of the LTCC may recover from inactivation within a single beat during the AP plateau (Acsai et al., 2011). The local feedback of Ca²⁺ on LTCC may contribute to the intrinsic BVR of the AP in normal physiology (Antoons et al., 2015). Interestingly, the same study did not find a major role for the NCX in BVR, although immunohistochemistry and functional studies have suggested colocalization of a fraction of NCX with LTCC (10–15% of total NCX) sensing local Ca²⁺ release in the dyadic subspace (Acsai et al., 2011; Scriven and Moore, 2013). In support of this notion, modulation of (dyadic) Ca²⁺ sparks by both reverse mode and forward mode NCX has been demonstrated (Neco et al., 2010; Biesmans et al., 2011).

To regulate CICR, the dyad harbors a repertoire of kinases and phosphatases that form macromolecular complexes with LTCC and RyR and regulate their levels of phosphorylation. PKA and CaMKII are key to the regulation of LTCC and RyR in the β-adrenergic and stretch response. PKA is targeted to LTCC and RyR via AKAPs, and transmits signals from β-ARs via cAMP (Catterall, 2015; Landstrom et al., 2017). Dyadic cAMP signals in the vicinity of LTCC and RyR are controlled by localized PDE activity (Kokkonen and Kass, 2017). It should be noted that exact mechanisms behind PKA regulation of RyR and its specific role in the β-adrenergic response are incompletely understood. Marx et al. (2000) proposed that PKA phosphorylation dissociates FKBP12.6 from RyR thereby enhancing RyR open probability. However, this mechanism remains questionable (Xiao et al., 2004). CaMKII is dually activated by Ca²⁺ and ROS (Maier and Bers, 2007), and possibly also by NO at high Ca²⁺ levels during β-adrenergic stimulation (Curran et al., 2014). Although CaMKII is targeted to both coupled and non-coupled RyR via unknown mechanisms, its activation is confined to the dyad, where it enhances the open probability of RyR and LTCC (Wehrens et al., 2004; Bers and Morotti, 2014).

Reactive Oxygen Species

In addition to phosphorylation mechanisms, ROS and NO have emerged as critical regulators of CICR. They modify LTCC and RyR function through redox modification of free cysteine residues. The action of ROS and NO is often multiphasic and bidirectional, depending on source, oxidant species, amount and timing and importantly, the local redox environment (Zima and Blatter, 2006). Typically, free radicals are short-lived and can only act on effectors in the close vicinity. Thus, redox modulation of Ca²⁺ in a cardiac myocyte is basically a tale of microdomain signaling of which the specific effects are determined by the subcellular location of the ROS/NO source and co-localization with its target proteins. Endogenous ROS are generated in the mitochondria as a by-product of respiration, and locally in the cytosol by specialized enzymes, such as NADPH oxidases (Burgoyne et al., 2012). Much of the O₂^- produced is rapidly converted to H₂O₂, a more stable and membrane permeable derivative. Endogenous NO is produced in relatively low concentrations by endothelial and neuronal isoforms of NOS (eNOS and nNOS, respectively) (Massion et al., 2003).

An important player in the redox control of dyadic Ca²⁺ is NOX2, a membrane-bound NADPH oxidase that resides in T-tubules. NOX2 is induced by fast pacing and stretch, and activates RyR via S-glutathionylation (Sánchez et al., 2005). RyR activation by rapid pacing also requires CaMKII, which itself is redox regulated (Erickson et al., 2008). Interestingly, the NOX2-CaMKII regulation of RyR is restricted to the dyadic cleft. In pig myocytes that resemble human and have a significant population of non-coupled RyR, faster pacing significantly increased Ca²⁺ spark activity of dyadic RyR, but not the activity of non-coupled RyR. Additionally, NOX2 and CaMKII inhibition abolished Ca²⁺ sparks in dyadic regions, but not near non-coupled regions (Dries et al., 2013). At this point it cannot be concluded if NOX2-derived ROS is upstream of CaMKII oxidation [as suggested in models of oxidative stress induced by angiotensin (Erickson et al., 2008; Purohit et al., 2013)] or whether NOX2 and CaMKII act in parallel. Furthermore, exact mechanisms of microdomain-specific activation of NOX2 and CaMKII in response to rapid pacing remain elusive. NOX2 is also activated by stretch. Prosser et al. (2011) have demonstrated that stretching a myocyte triggered an immediate burst of ROS and Ca²⁺ sparks. The ROS was derived from NOX2 as the response was sensitive to NOX2 inhibitors and absent in NOX2 deficient mice (Prosser et al., 2011).

In contrast to NOX2, mitochondria constitutively produce ROS. Mitochondria are located at a very short distance of dyadic regions [between 37 and 270 nm based on electron microscopy analysis of rat myocardium (Sharma et al., 2000)]. Several studies have shown that mitochondrial ROS can activate RyR (reviewed in Zhang H. et al., 2013), suggesting that basal ROS production by mitochondria is responsible for a significant portion of spontaneous Ca²⁺ sparks (Yan et al., 2008).

The LTCC also acts as a redox sensor due to free thiol groups in its α1-subunit (Muralidharan et al., 2016). Reported ROS effects on Ca²⁺ channel function are both stimulatory (Song et al., 2010), or inhibitory (Gill et al., 1995). This discrepancy might be due to differences in the phosphorylation state of the Ca²⁺ channel. Several serine/threonine kinases that regulate the channel are subjected to ROS modification, including PKA, PKC, and CaMKII (see Burgoyne et al., 2012, for review). The positive effects of phosphorylation might partially counterbalance the inhibitory effects of direct ROS oxidation of LTCC. During high oxidative stress, LTCC facilitation by CaMKII is likely the predominant effect, since the calcium antagonist nifedipine could suppress the induction of EADs by H₂O₂ (Xie et al., 2009). ROS regulates many other proteins of the Ca²⁺ machinery outside the dyad. The overall effect of sustained ROS is Na⁺ and Ca²⁺ overload promoting even more ROS production via positive feedback and predisposing the cell to afterdepolarizations (Wagner et al., 2013).

Nitric oxide is both a positive and a negative regulator of excitation–contraction (EC) coupling underscoring the complexity of cardiac NO signaling (Simon et al., 2014; Farah et al., 2018). NO exerts its action via two pathways: an indirect pathway by the activation of sGC producing cGMP, and a direct pathway by S-nitrosylation of proteins. High levels of NO would predominantly stimulate the cGMP pathway causing negative inotropy, while low levels activate nitrosylation processes leading to positive inotropy (González et al., 2008). The mechanisms of nitrosylation, and particularly its effects on EC coupling, remain poorly understood. Despite much controversy, some consensus has emerged on the specific roles of eNOS and nNOS highlighting the importance of their subcellular localization in modulating Ca²⁺ handling proteins. Colocalization of eNOS and LTCC in caveolae at the sarcolemma favors S-nitrosylation and inhibition of LTCC (Wang et al., 2008). nNOS is targeted to the SR where it colocalizes with RyR and is therefore considered the prime NO modulator of dyadic Ca²⁺ (Barouch et al., 2002). NO nitrosylates RyR and increases its activity (Wang et al., 2010, but see also Zahradníková et al., 1997). The notion that the positive effects of nNOS are linked to its specific localization on the SR has been supported by a recent study that developed a transgenic mouse model in which nNOS was targeted to the sarcolemma and no longer co-localized with RyR (Carnicer et al., 2017). Interestingly, relocalization of nNOS, as may occur in HF, produced the same negative effects on I_CaL and contraction as eNOS. In normal physiology, NOS activity is controlled by β-adrenergic stimulation and stretch, as will be discussed in the next paragraphs.

β-Adrenergic Signaling and Afterdepolarizations

In the heart, enhanced sympathetic activity is a potent stimulus for generation of arrhythmias. The relationship between sympathetic stimulation and triggered activity has long been recognized, in vivo (Priori et al., 1988, 1990) and in vitro (Lazzara and Marchi, 1989). During β-adrenergic stimulation, DADs and EADs often coexist. When Ca²⁺ overload plays a role in afterdepolarization formation, as could be the case under β-adrenergic stimulation, both EADs and DADs can be abolished by ryanodine, suggesting a common dependence of these on SR Ca²⁺ release under these conditions. In cardiac myocytes, β-ARs and their effector pathways targeting Ca²⁺ handling proteins are highly compartmentalized. In this paragraph, we will discuss the parallel activation of multiple molecular pathways by β-adrenergic subtypes, their specific end targets to controlling local and global Ca²⁺ release, and their impact on the generation of DADs and EADs.

β-Adrenergic Signaling Pathway

β-Adrenergic stimulation activates two pathways that operate in parallel: a PKA-dependent pathway that impacts on systolic Ca²⁺ through modulation of SR Ca²⁺ load, and a CaMKII pathway that regulates diastolic SR Ca²⁺ release (Figure 2A). Molecularly, the PKA signaling cascade is clearly defined. Upon activation, β-adrenergic agonists stimulate adenylate cyclase via Gs-coupled proteins raising cAMP levels that activates PKA (Bers, 2002). Subsequent phosphorylation of PKA substrates, including LTCC (causing increased Ca²⁺ influx) and PLB (accelerating SR Ca²⁺ uptake), results in enhancement of SR Ca²⁺ load. In cardiac myocytes, the compartmentation of cAMP signaling has been attributed to different β-AR subtypes that have distinct subcellular locations. β2 receptors are preferentially located at T-tubules where they co-localize with LTCC in caveolae, while β1 receptors are distributed more globally across T-tubules and surface sarcolemma. Using FRET sensors for cAMP, it was demonstrated that selective β1 stimulation generates cAMP signals that propagate throughout the cell, whereas the β2 AR signal is locally confined in T-tubules and specifically regulates LTCC during CICR (Nikolaev et al., 2006).

FIGURE 2.

Proposed pathways for inducing SR Ca²⁺ leak during β-adrenergic signaling and stretch in a ventricular cardiomyocyte. (A) β-AR raises cAMP levels via G_s-protein-dependent activation of AC that activates both PKA and Epac. PKA phosphorylates LTCC and PLB leading to more Ca²⁺ influx and faster uptake by SERCA into the SR. Epac activates nNOS and CaMKII via an PI3K and AkT signaling cascade promoting SR Ca²⁺ leak via RyR phosphorylation. The broken line indicates a cAMP and Epac-independent pathway for local activation of nNOS targeted to RyR in the dyad. RyR not coupled to LTCC in T-tubules are not modulated by CaMKII and nNOS. eNOS is localized to caveolae and exerts negative effects on LTCC during β-adrenergic stimulation. (B) Mechanotransduction involves ROS and NO for RyR activation. The ROS and NO pathway are independent and operate on different timescales via different mechanosensors. NOX2 produces ROS near RyR in the dyad increasing RyR activity possibly via oxidation of CaMKII. With a delay, nNOS is activated via an unknown mechanosensing mechanism. The enhanced SR Ca²⁺ leak promotes Ca²⁺ waves that activate a transient inward NCX current causing DAD. Caveolar eNOS is activated by stretch via PI3K-Akt and positively modulates EC coupling outside the dyad by mechanisms that are incompletely understood. See text for further details. AC, adenyl cyclase; β-AR, β-adrenergic receptor; cAMP, cyclic adenosine 3’,5’-monophosphate; CaMKII, Ca²⁺/calmodulin-dependent protein kinase II; EC, excitation contraction; DAD, delayed afterdepolarizations; eNOS, endothelial nitric oxide synthase; Epac, exchange protein activated by cAMP; LTCC, L-type Ca channel; NCX, Na⁺/Ca²⁺ exchanger; NO, nitric oxide; NOX2, NADPH oxidase type 2; nNOS, neuronal nitric oxide synthase; PI3K, phosphoinositide 3-kinase; PKA, protein kinase A; PLB, phospholamban; ROS, reactive oxygen species; RyR, ryanodine receptor; SERCA, SR Ca²⁺-ATPase; SR, sarcoplasmic reticulum.

In vitro, PKA can also phosphorylate RyR (Marx et al., 2000), but in intact myocytes a functional role for PKA regulation of RyR remains controversial. Most evidence is in favor of CaMKII as the prime modulator of RyR during β-adrenergic stimulation. In response to adrenergic activation, CaMKII phosphorylates RyR and increases open probability when measured as SR Ca²⁺ leak (Curran et al., 2007) or diastolic Ca²⁺ sparks (Gutierrez et al., 2013). A recent study in pig myocytes suggested that CaMKII-dependent modulation of RyR during β-adrenergic stimulation was restricted to RyRs specifically in the dyadic cleft, and involved local activation of nNOS (Dries et al., 2016). The nNOS/CaMKII effects were not observed in RyR release sites that were not coupled to T-tubules. While there is conclusive evidence to support nNOS involvement in β-adrenergic modulation of RyR (Massion et al., 2003), the steps upstream from nNOS activation are less well defined. Some, but not all, studies suggested the involvement of Epac, a cAMP target parallel to PKA, leading to CaMKII autophosphorylation and downstream RyR phosphorylation (Pereira et al., 2007; Oestreich et al., 2009). A second pathway, independent of cAMP, involves PI3K and Akt as upstream activators of nNOS inducing CaMKII activation via nitrosylation (Curran et al., 2014). A recent study suggested that the Epac and nNOS pathway are interdependent and function largely in series (Pereira et al., 2017).

During β-adrenergic stimulation, the cAMP-PKA and nNOS-CaMKII pathways operate in parallel. From the current evidence it is clear that PKA signaling is distributed more globally with robust effects on LTCC and SERCA, whereas CaMKII is highly localized and exerts stronger effects than PKA on RyR. Ca²⁺ current facilitation by CaMKII under β-AR has also been demonstrated (Dries et al., 2016). The integrated response is enhancement of LTCC currents, SR Ca²⁺ load and leak. Together, these effects are able to favor both EADs and DADs.

Ca²⁺-Dependent Mechanisms of Afterdepolarizations During β-Adrenergic Stimulation

The link between β-adrenergic stimulation, RyR-mediated Ca²⁺ leak and arrhythmogenesis has been most convincingly demonstrated in the clinical case of catecholaminergic polymorphic ventricular tachycardia (CPVT). Patients with CPVT carry mutations in RyR that increase the open probability of the receptor, or in calsequestrin (Casq2) where SR Ca²⁺ buffering is hindered and/or the interaction of Casq2 and RyR is altered (Cerrone et al., 2009). Introducing a CPVT associated RyR mutation in a mouse model, for example, resulted in a higher rate of Ca²⁺ sparks, waves and DADs in myocytes, and development of bidirectional ventricular tachycardia after exposure to catecholamines (Cerrone et al., 2005; Liu et al., 2006; Fernández-Velasco et al., 2009). The higher incidence of DADs and triggered activity has been attributed to increased RyR sensitization to Ca²⁺ (lowering the SR threshold for Ca²⁺ waves), and the enhanced SR Ca²⁺ by catecholamines (Fernández-Velasco et al., 2009; Kashimura et al., 2010). DAD occurrence also critically depends on the balance between SR Ca²⁺ load and the diastolic interval. Short durations reduce the time for SR refilling and recovery, and hence the likelihood of DADs. CPVT patients sometimes develop bradycardia. In this population the slow supraventricular rate has been suggested as the primary cause of ventricular arrhythmias (Faggioni et al., 2013), which may explain the reduced response of some patients to β-blockers (Priori et al., 2002). During bradycardia, particularly the Purkinje cells of the conduction system are prone to developing DADs and present a major source of focal activity in CPVT (Cerrone et al., 2007).

In conditions of excessive Ca²⁺ load, DADs and EADs often appear simultaneously (Priori and Corr, 1990; Volders et al., 1997; Antoons et al., 2007). A study by Johnson et al. (2013) in dog ventricular myocytes proposed a mechanism that coupled diastolic DADs to increased BVR and EADs. In the presence of isoproterenol, diastolic Ca²⁺ waves and DADs frequently appeared between beats. After a DAD, the duration of the next AP was consistently prolonged, related to an increase in I_CaL. The Ca transient during CICR was smaller after a DAD (presumably due to partial SR depletion), and modeling and voltage-clamp analysis explained the I_CaL facilitation by a reduction of Ca²⁺ dependent inactivation of I_CaL. The feedback of DAD on I_CaL caused significant BVR. Furthermore, because of the prolonged AP after a DAD, new Ca²⁺ waves could be generated before the end of repolarization and form EADs.

The phenomenal observation of Ca²⁺ sparks and waves that occur due to spontaneous (not triggered) openings of RyR clusters during diastole is well known (Cheng and Lederer, 2008). Triggered Ca²⁺ sparks, i.e., produced by LTCC openings during CICR, occur near-synchronously at the start of the Ca²⁺ transient and are not expected to occur during relaxation because of refractoriness of the RyR and SR Ca²⁺ content. However, using high sensitivity detectors during confocal imaging in rabbit ventricular myocytes, Fowler et al. (2018) detected Ca²⁺ sparks during the decay of the Ca²⁺ transient. They explained the occurrence of these late Ca²⁺ sparks by the ability of release sites to recover from refractoriness during the plateau phase of the AP to become reactivated either by cytosolic Ca²⁺ itself, or by stochastic openings of LTCC. Late Ca²⁺ sparks are more readily observed when CaMKII activity is increased, as was reported in a mouse model of CaMKIIδc overexpression (Guo et al., 2012). CaMKII phosphorylation of LTCC causes a shift in the distribution of LTCC into high-activity gating modes and accelerates recovery from inactivation (Sham et al., 1995; Guo and Duff, 2006), which could explain the facilitation of late Ca²⁺ sparks by CaMKII. Interestingly, repetitively firing of late Ca²⁺ sparks produced microscopic waves of Ca²⁺ release presenting a new paradigm of electrical instability underlying BVR and EAD (induced by a DAD-like mechanism during the AP plateau), particularly in settings of HF with prolonged AP and increased CaMKII activity.

A final mechanism of EAD seen with β-AR relates to the dynamic modulation of Ca²⁺ window currents through Ca²⁺-dependent feedback. The mechanism is independent of spontaneous Ca²⁺ events (unlike the mechanisms discussed above) but reflects local feedback of SR Ca²⁺ release on LTCC during CICR. In the classical view, EAD are caused by voltage-dependent recovery of inactivated LTCC. Inactivation and recovery of LTCC also have a Ca²⁺-dependent component (Sipido et al., 1995; Sham, 1997), dynamically shaping Ca²⁺ window currents during a single beat. In dog and pig myocytes, we observed that under isoproterenol Ca²⁺-dependent recovery of window currents was faster than the decay of global Ca²⁺ transients, relatively unaffected by slow Ca²⁺ buffers and absent when SR Ca²⁺ release was inhibited (Antoons et al., 2007; Acsai et al., 2011). These data strongly suggest that release-dependent recovery of window currents is driven by local changes in dyadic Ca²⁺. The Ca²⁺-dependent regulation of Ca²⁺ window currents seem to require sufficiently high levels of dyadic Ca²⁺, or local activation of CaMKII, as the dynamic inactivation and recovery process was no longer observed in the absence of isoproterenol. Enhanced dynamic modulation of window LTCC by dyadic Ca²⁺ release is a suggested source of BVR (Antoons et al., 2015), and may contribute to an increased incidence of EADs under β-AR stimulation (Antoons et al., 2007).

Mechanical Load and Afterdepolarizations

Acute stretching of the heart destabilizes membrane potential and causes DADs and EADs (Franz et al., 1989). This arrhythmogenic activity is caused by negative feedback mechanisms that integrate mechanical and electrical activity of cardiac myocytes and presumably involve Ca²⁺ (Ravens, 2003). The myocardium responds to stretch by a more powerful contraction, a phenomenon referred to by Frank Starling (Sagawa et al., 1990). The intrinsic adaptation to changes in mechanical load has a second slower component of enhanced contractility described by the Anrep effect (von Anrep, 1912). Early work in intact cardiac muscle had not observed significant changes in diastolic and systolic global Ca²⁺ levels during the initial stretch response (Allen and Kurihara, 1982), which has argued against a major role for Ca²⁺ in stretch-induced arrhythmias. More recently, this view has been challenged by experiments that confocally monitored Ca²⁺ sparks and waves during stretch, suggesting that local Ca²⁺ release could account for at least part of the Frank Starling response (Petroff et al., 2001). Since then, a complex picture of mechanosensitive Ca²⁺ signaling has emerged that operates over a wide range of time scales. Within milliseconds, a small diastolic stretch triggers a burst of Ca²⁺ sparks (Iribe et al., 2009). When sustained, Ca²⁺ accumulates over minutes via stretch-induced autocrine/paracrine signaling participating in the Anrep effect (Cingolani et al., 2013). When stress becomes chronic, elevated Ca²⁺ influx activates gene expression leading to hypertrophy (Tavi et al., 2001; Gómez et al., 2013).

RyR Mechanosensitivity

Stretch-dependent regulation of the Ca²⁺ system is operated via the process of mechanotransduction. Its mechanisms involve many signaling cascades targeting a diversity of intracellular Ca²⁺ sources, including the SR and mitochondria (Schönleitner et al., 2017). Furthermore, mechanotransduction operates via different classes of mechanosensors of which the activation seems to depend on the mechanical environment of the myocyte, which in experimental settings is defined by the dimensionality of the stretch system (Chen-Izu and Izu, 2017). The modulation of RyR by mechanical force has been a focus of investigation after the first demonstration in a 3D cell-in-gel system that stretch can trigger Ca²⁺ sparks (Petroff et al., 2001). Subsequently, ROS and NO have been identified as key molecules in RyR mechanosensitivity (Figure 2B). NOX2 activation has been proposed as the principle mechanosensor underlying the initial fast response of Ca²⁺ sparks to stretch (Prosser et al., 2011). Stretch-induced ROS by NOX2 is fast, transient and confined near the dyad to permit rapid and reversible modification of RyR. It is therefore believed that the NOX2 pathway enhances CICR efficiency without changes in systolic Ca²⁺ and serves as an adaptation to beat-to-beat variations in preload contributing to the Frank Starling response.

Cardiac stretch also stimulates cardiomyocytes to produce NO (Khairallah et al., 2012). Mechanical stimulation of NO elevates the systolic Ca²⁺ transient and produces spontaneous Ca²⁺ sparks during diastole, as was demonstrated in myocytes contracting in-gel against a higher preload or afterload (Petroff et al., 2001; Jian et al., 2014). In these settings, NO was produced through activation of constitutive NOS by phosphorylation via the PI3K-Akt pathway (Petroff et al., 2001). Pharmacological inhibition or genetic deletion to differentiate between eNOS and nNOS pathways revealed that both isoforms were involved in the downstream effects on systolic Ca²⁺, but only nNOS had a role in the induction of Ca²⁺ sparks (Jian et al., 2014). The divergent effects of eNOS and nNOS have been explained by their subcellular location. nNOS is localized at the dyad in close proximity of RyR, while eNOS is spatially confined in caveolae more distant from RyR release sites (Xu et al., 1999). Downstream from nNOS signaling, CaMKII was also found to modulate afterload-induced Ca²⁺ sparks. The mechanically induced SR Ca²⁺ leak by nNOS is expected to deplete the SR of Ca²⁺, however, SR Ca²⁺ content is maintained presumably via enhanced SERCA Ca²⁺ reuptake by nNOS (Vielma et al., 2016).

The NO-mediated increase of the Ca²⁺ transient to compensate for greater mechanical load typically appears with a delay of seconds and minutes, possibly participating in the Anrep effect. Trans-sarcolemmal Ca²⁺ influx also contributes to the slow Ca²⁺ loading during stretch. One of the proposed mechanisms is increased activity of Na⁺/H⁺ exchanger through mitochondrial ROS release downstream of stretch-induced angiotensin signaling (Cingolani et al., 2013). The result is an increase in intracellular Na⁺ that stimulates reverse NCX loading the cell with Ca²⁺. Na⁺ and Ca²⁺ influx through non-selective cationic SAC may further contribute (Calaghan et al., 2004). Thus, slow adaptation to stretch is viewed as an enduring signal achieved by concerted action of local nNOS activity to fine-tune local Ca²⁺ release and transsarcolemmal Ca²⁺ and Na⁺ influx to gain more Ca²⁺.

Ca²⁺-Dependent Mechanisms of Stretch-Induced Arrhythmias

While mechano-sensitization of RyR is part of an effective adaptation to preload and afterload by increasing the efficiency of local Ca²⁺ release, it also produces spontaneous Ca²⁺ sparks during diastole. In the normal heart, stretch-induced Ca²⁺ sparks are locally confined. Under certain conditions, when more Ca²⁺ sparks arise synchronously to form Ca²⁺ waves, the load-adaptive Ca²⁺ system could turn into an arrhythmogenic mechanism. The stretch-induced increase in ROS, Ca²⁺ sparks and velocity of propagating Ca²⁺ waves is graded, i.e., increases with increasing amount of stretch (Miura et al., 2015). Thus, large stretches, such as in dilated hearts, are more likely to trigger ventricular ectopy (Hansen et al., 1990). Mechanical dyssynchrony, often due to structural tissue heterogeneity, is a further compromising factor. In case of dyssynchronous contractions, Ca²⁺ waves arise from a non-SR source as result from Ca²⁺-dissociation from the contractile filaments during late relaxation of the non-uniform cardiac muscle (Miura et al., 2008).

More ROS can also by produced by hypersensitivity of mechanosensitive signaling due to upregulation of a molecular component, as was demonstrated for a mouse model of Duchenne muscular dystrophy that showed upregulation of NOX2 and produced Ca²⁺ waves in response to moderate stretch (Prosser et al., 2011). In addition to DADs, ROS also activates EADs, via reactivation of I_CaL (Song et al., 2010), or enhanced late Na⁺ current (Song Y. et al., 2006). While RyR, INaL, and LTCC can be directly activated by oxidation (Xu et al., 1998; Morita et al., 2003; Kassmann et al., 2008), redox modification of CaMKII seems to be crucially involved in ROS modulation of arrhythmogenic INaL and LTCC currents (Morita et al., 2009; Wagner et al., 2011). Of note, most electrophysiology studies applied H₂O₂ as an exogenous source of ROS. There are no current data to confirm if endogenous ROS produced by NOX2 during stretch behaves similarly. Source matters, as mitochondrial ROS caused a reduction of INa (Liu et al., 2010).

While ROS is a ubiquitous proarrhythmic signal, NO generate opposite pro- and antiarrhythmic signals that can be partly explained by divergent effects of eNOS and nNOS on Ca²⁺ handling proteins. Mice with targeted disruption of eNOS had a higher incidence of arrhythmias induced by ouabain (Rakhit et al., 2001) or β-adrenergic stimulation (Wang et al., 2008), confirming earlier work reporting on the protective effects of NO against ventricular arrhythmias in dogs (Vegh et al., 1992). The antiarrhythmic effects have been attributed to β-adrenergic antagonism of eNOS via reduction of I_CaL in a cGMP-dependent manner. Likewise, nNOS knockout mice suffered more from arrhythmias after myocardial infarction than their WT littermates. Because an I_CaL blocker reduced VF incidence, the authors concluded that nNOS is antiarrhythmic through I_CaL inhibition via direct nitrosylation (Burger et al., 2009). Nitrosylation of the Na⁺ channel is also coupled to nNOS activity (Ahern et al., 2000). Conversely, when nNOS is activated by stretch or catecholamines in cardiac myocytes, local NO-CaMKII signals produce arrhythmogenic Ca²⁺ waves that originate from dyadic RyR (Curran et al., 2014; Jian et al., 2014). Giving the pro-arrhythmic actions of isoproterenol in vivo, it is reasonable to argue that during β-adrenergic stimulation in the presence of mechanical load, pro-arrhythmogenic nNOS signaling prevails.

In unloaded myocytes, the I_TI following a Ca²⁺ wave is mainly produced by NCX. In stretched myocytes, a significant contribution of stretch-activated channels is anticipated. Stretch-activated non-selective cation currents (SACNS) have been functionally demonstrated in ventricular myocytes at the whole-cell and single-level (Craelius et al., 1988). While it is unlikely that Na⁺ and Ca²⁺ conducting SACNS participate in stretch-induced SR Ca²⁺ release in ventricular myocytes (Iribe et al., 2009), they may contribute to destabilize the resting membrane potential by generating inward current during diastole. Studies in whole hearts demonstrating anti-arrhythmic effects of GsMTx-4, a specific SACNS blocker, support the involvement of SAC in stretch-induced arrhythmias (Wang et al., 2016). The search for a ‘true’ SACNS, a structural homolog to the bacterial SAC that can be directly gated by membrane tension (Sukharev et al., 1994), is still ongoing. Interestingly in this regard is the discovery of Piezo channels in a neuroblastoma cell line (Coste et al., 2010). The biophysiological profile of Piezo matches endogenous cardiac SACNS, including weak voltage dependency, single channel conductance, inactivation, and sensitivity to GsMTx-4 (Gottlieb, 2017), and is therefore a promising candidate. Piezo is expressed at low levels in the heart (Coste et al., 2010), but its role in cardiac function has yet to be established.

In the heart, the search for cardiac SACNS has been largely focused on the transient receptor potential canonical (TRPC) channel family. The activation of TRP channels is polymodal, and some members are directly activated by membrane deformation (Inoue et al., 2009), although this remains somewhat controversial (Gottlieb et al., 2008). Two subtypes, TRPC3 and TRPC6, have been proposed as potential candidates participating in the slow force response (Yamaguchi et al., 2017). Hyperactive TRPC3 (Doleschal et al., 2015) or TRPC6 (Seo et al., 2014) amplified the slow inotropic response to stretch resulting in Ca²⁺ overload and arrhythmogenesis. Doleschal et al. (2015) explained the pro-arrhythmia of TRPC3 by a Ca²⁺ overload dependent mechanism that involves spatial uncoupling between TRPC3 and NCX1 in specialized microdomains disrupting the tight regulation of NCX by local Ca²⁺ and Na⁺. This thinking is in line with the conceptual view that TRPC channels have access to localized Ca²⁺ signaling microdomains that are separated from contractile dyadic signaling (Houser and Molkentin, 2008). The microdomain concept was initially proposed to explain the role of TRPC channels in the activation of the NFAT/calcineurin axis linking pathophysiological hypertrophy to chronic mechanical stretch (Kuwahara et al., 2006; Makarewich et al., 2014). It has been well established that structural and functional remodeling in pathological cardiovascular stress predisposes the heart to arrhythmias (Nattel et al., 2007; Orini et al., 2017).

Linking Mechanotransduction and Adrenergic Signaling

Thus far, ROS, NO, and CaMKII have been identified as the prime mediators of RyR mechanosensitivity in the intrinsic adaptation of contractile force to load. In vivo, intrinsic force adaptation is modulated by sympathetic activation by imposing a higher load on the heart through modulation of vascular tone. The myocyte can respond to higher mechanical and adrenergic stress through activation of mechanotransduction and adrenergic signaling networks, as discussed above and depicted in Figure 2, but interactions have not been systematically investigated. The mechanotransduction pathway shows both the rapid preload-induced NOX2 and slower afterload-induced NO branch, that most probably operate independently (Jian et al., 2014). It is also unlikely that NOX2 is directly involved in β-adrenergic signaling, since ROS scavengers failed to prevent increases in Ca²⁺ spark frequency in quiescent cells that were treated with isoproterenol (Gutierrez et al., 2013). It should be noted that NOX2 can possibly become activated during β-adrenergic signaling as an indirect consequence of chronotropic effects (Dries et al., 2013).

nNOS is centrally involved in both stretch- and adrenergically induced Ca²⁺ sparks, most likely via oxidation of downstream CaMKII (Gutierrez et al., 2013; Jian et al., 2014). It is therefore tempting to speculate that nNOS and CaMKII act as primary integrators of mechanotransduction and adrenergic RyR signaling networks. The assumption that co-activation of nNOS has a cumulative effect on RyR activity remains to be determined.

The eNOS effects are less clearly defined. eNOS is compartmentalized in caveolae at T-tubules and sarcolemma. In sarcolemmal caveolae, eNOS colocalizes with β-ARs and LTCC allowing NO to mitigate β-adrenergic inotropy through inhibition of LTCC by local cGMP (Wang et al., 2008). It is conceivable that a stretch activation of the eNOS-Akt-PI3K pathway positively modulates EC coupling gain in T-tubular caveolae, while negatively regulating the β-adrenergic response in a different subset of caveolae at the surface sarcolemma.

Heart Failure and Afterdepolarizations

Heart failure is associated with extensive cardiac remodeling, at both the structural and functional levels. Remodeling due to HF occurs for a number of reasons, however, it is in part, due to altered stress on the ventricular wall (Kehat and Molkentin, 2010).

Remodeling can lead to an increased propensity for complex ventricular arrhythmias and sudden cardiac death, and these are seen in over half of the patients presenting with HF with reduced ejection fraction. For these reasons it is imperative to understand the mechanisms that are responsible for the increased arrhythmia incidence in this population (Janse, 2004).

Purkinje fibers isolated from infarcted sections of human hearts have been shown to have significantly longer APD than those from non-infarcted zones, resulting in marked dispersion of APD in infarcted and adjacent zones. Furthermore, both epinephrine and the cardiac glycoside, ouabain, were able to induce DADs in these fibers (Dangman et al., 1982). Previous work using human trabeculae has also shown that there is an increased propensity for triggered activity in tissue from HF patients (Vermeulen et al., 1994). Further work from the Amsterdam group also showed that, in contrast to many animal species, norepinephrine induces APD prolongation in ventricular myocytes from human failing hearts, as well as EADs. These alterations were ascribed to an increase in both the calcium peak current and window current (Veldkamp et al., 2001).

In addition to the alterations in arrhythmia incidence in HF, it has been well described that the failing heart has a reduced responsiveness to elevated catecholamine levels, at least in end-stage HF, due to alterations in expression of β-ARs (Bristow et al., 1982; Ungerer et al., 1993). Interestingly, more recent work has also shown that in a patient cohort with HF, BVR of ventricular AP duration was increased during an autonomic challenge associated with increased sympathetic activity (Porter et al., 2017).

Taking these data together leads us to believe that modifications in signaling underlying β-adrenergic responsiveness and stretch may contribute to the increased occurrence of arrhythmias in these patient populations. Therefore, if we are able to understand the precise changes that occur in these systems during HF, we may get a better hold on the processed occurring, with an outlook of preventing and/or treating the, potentially, maladaptive remodeling (see below).

Global Remodeling

At the gross structural level, the geometry of the heart changes as a result of HF, becoming less elliptical and more spherical (Cohn et al., 2000). HF is associated with a progressive enlargement of the left ventricle, with increases in end-systolic left ventricular wall stress being seen (Florea et al., 1999), which may have detrimental effects on mechanosensitive mechanisms involved in arrhythmia formation, and also contribute to the cellular arrhythmogenic remodeling.

Ion Channel Remodeling

At the level of the single myocyte, changes in HF include alterations in the densities of various membrane channels, which contributes to the increase in APD seen in the majority of HF models and in patients (Beuckelmann et al., 1993; Tomaselli and Marbán, 1999). One of the most consistent findings with regards to current alterations in HF is the decrease in the inwardly rectifying potassium current, I_K1, which contributes to maintaining the resting membrane potential as well as contributing to terminal repolarization (Beuckelmann et al., 1993; Nerbonne and Kass, 2005). Furthermore, the β-adrenergic regulation of I_K1 has also been shown to be significantly reduced in myocytes isolated from HF patients (Koumi et al., 1995). Reduced I_K1, will mean that a smaller I_TI will be required to cause the same amplitude of DAD, or even triggered AP, and therefore altered regulation of this current in HF has major implications in the potential arrhythmogenic outcomes. An interesting recent study, however, showed that sympathetically -induced arrhythmias could not be induced when I_K1 was inhibited in isolation in Langendorff-perfused rabbit hearts indicating that synergistic activity between multiple pathways, including altered RyR sensitivity, was required for arrhythmia induction (Myles et al., 2015).

Another potassium current that is of great interest when it comes to β-adrenergic modulation, is the slow rectifier, I_Ks. I_Ks function is prominent during β-adrenergic stimulation when it promotes AP shortening, to counteract the increase in inward Ca²⁺ current, thus providing critical “reserve” when other repolarizing currents are impaired (Jost et al., 2005; Varró et al., 2000; Volders et al., 2003). Although Veldkamp et al. (1995) could not detect this current in myocytes isolated from patients with cardiomyopathy, a number of animal models have indicated that this current is downregulated in HF (Tsuji et al., 2000; Li et al., 2002). A decrease in this current during intense sympathetic stimulation will lead to an increase in APD, and an increased tendency for afterdepolarizations. Furthermore, research from our own group has shown the key role that I_Ks plays in preventing excessive BVR during β-AR stimulation, which may also contribute to the arrhythmogenic substrate generated when this current is downregulated (Johnson et al., 2010, 2013).

The importance of both I_K1 and I_Ks and their regulation by β-adrenergic stimulation in HF were recently highlighted by a study from the Bers’ group. In this manuscript, the physiologically relevant AP-clamp technique was utilized to show that under β-adrenergic stimulation, reduced I_Ks responsiveness limits the integrated repolarizing potassium currents in a rabbit model of HF. Furthermore, an increase in APD BVR was seen in HF myocytes. Taken together these data illustrate the importance that these currents may play in arrhythmia generation in HF, especially under sympathetic stimulation (Hegyi et al., 2018a).

Apart from the acute effects of adrenergic stimulation on channel activity, one must also consider the effect of sustained sympathetic activation. A recent study did just this by investigating the effects of sustained adrenergic stimulation on I_Ks dynamics. In that particular study, they showed that I_Ks was reduced after continued β-AR stimulation, and this was mediated by CaMKII, a signaling molecule involved in both β-AR and mechanosensitive stimulated arrhythmias (Shugg et al., 2018). If this effect contributes to the increased incidence of arrhythmias in HF is currently unknown and should be the subject of further work.

With regards to the acute effect of HF on SACNS, that may also contribute to stretch-induced arrhythmias, multiple laboratories have shown that TRPC channel expression and activity are upregulated in pathological hypertrophy and HF (Eder and Molkentin, 2011). Furthermore, to our knowledge, to date only one study has investigated the level of Piezo channels in HF, with that study providing evidence of an upregulation in HF. However, the functional consequences of this upregulation are currently unknown and should be the subject of further research (Liang et al., 2017).

Excitation–Contraction Coupling Remodeling

As previously stated, the synchronous rises in Ca²⁺ leading to efficient ECC is due, in part, to the tight opposition of RyRs and LTCCs in the T-tubules in healthy ventricular myocytes. There is an abundance of literature describing a loss of T-tubules during HF (Lyon et al., 2009; Guo et al., 2013; Dries et al., 2018a), In addition to the loss of the concerted effort for successful ECC that the loss of T-tubules will bring, deleterious Ca²⁺ handling leading to arrhythmia may also result. A recent study investigated the potential mechanisms behind T-tubule disruption in post-infarction failing rat hearts. In that study, they showed that elevated wall stress was associated with disruption of the T-tubular structure and this was associated with decreased levels of junctophilin 2, which is a critical dyadic anchor. Furthermore, they carried out studies on loaded papillary muscles, which confirmed a direct role of wall stress on regulation of T-tubule organization (Frisk et al., 2016). Taken together these data indicate the importance that stretch has in developing the HF phenotype when it comes to subcellular structure of the myocyte. Alterations in location of relevant signaling pathways that may also be induced by this loss of cellular architecture will be discussed later (see the Section “Local (Microdomain) Remodeling”).

As the current generated via the NCX appears to be the major player responsible for the I_TI that initiates DADs, and perhaps EADs, one also needs to consider how the function of this exchanger is altered in HF. Interestingly, a number of in vitro studies have suggested that stretch of adult myocytes increases NCX expression (Sipido et al., 2002). These data may lead us to believe that the increase stretch ‘felt’ by the in situ myocyte may also lead to an increase in NCX in HF. Indeed, the majority of studies have shown that NCX is increased in HF (Sipido et al., 2002; Schillinger et al., 2003), although we should approach these data with caution due to the fact that expression levels do not necessarily give an indication of activity, especially when considering an exchanger where ion concentrations, phosphorylation state [of partner proteins (e.g., phospholemman) as well as NCX itself] in addition to other factors will ultimately influence the current generated by the exchanger.

One of the major influences on NCX activity is the intracellular Ca²⁺ concentration. It is well known that cardiomyocytes isolated from failing hearts (with reduced ejection fraction) show altered Na⁺ and Ca²⁺ haemostasis. The modified Ca²⁺ handling is characterized by decreased Ca²⁺ transients, enhanced diastolic SR Ca²⁺ release and diminished SR Ca²⁺ reuptake, which all contribute to altered Ca²⁺ concentrations ‘seen’ by the NCX (Hasenfuss and Pieske, 2002; Kho et al., 2012; Luo and Anderson, 2013). Additionally, modeling studies have shown that that both dyadic and SR Ca²⁺ influence the appearance of DADs in addition to alterations in Ca²⁺ diffusion across the cell and Ca²⁺ uptake into the SR (Fink et al., 2011).

One of the first papers investigating Ca²⁺ sparks in myocytes from patients with HF indicated that alterations in the Ca²⁺ release mechanisms must be one of the mechanisms underlying EC coupling, in addition to alterations in SR Ca²⁺ load (Lindner et al., 2002). One of the driving forces behind this is the altered open probability of RyRs, which is governed by multiple factors, and has not been without controversy over the years (Dobrev and Wehrens, 2014). Interestingly, recent work has shown that stabilizing the RyR, using dantrolene, is able to prevent DADs in myocytes isolated from HF patients (Hartmann et al., 2017). Over the next few paragraphs, we will discuss how the major controllers of RyR stability, that also govern stretch and/or β-adrenergic signaling (namely CaMKII and ROS), can be affected in HF.

CaMKII phosphorylation of RyR appears to play an important role in arrhythmogenesis and sudden cardiac death in mice with HF (van Oort et al., 2010). Analysis from ventricular tissue from patients with either dilated or ischemic cardiomyopathy have shown that there is an increase in the levels of CaMKIIδ, the major isoform of CaMKII in the heart (Sossalla et al., 2010). Interestingly, single myocytes isolated from mice overexpressing CaMKIIδ are more liable to show DADs and spontaneous APs under β-adrenergic stimulation when compared to wild type mice (Sag et al., 2009). This increase in CaMKII seen in HF could directly promote arrhythmia formation by not only increasing diastolic Ca²⁺ leak via RyR phosphorylation, but also by promoting increases in the late Na⁺ current (Wagner et al., 2006), a current that has already been shown to be increased in HF, and incriminated in increased BVR and arrhythmia formation under these conditions (Maltsev et al., 2007).

The activity of CaMKII itself is under control of many different regulators, including ROS (as stated above and shown in Figure 2), which is detrimentally altered in HF. ROS also as having their own independent effects on RyR and other components of the Ca²⁺ handling machinery (dependent on the source of the ROS). In HF, just as in normal physiology, ROS has a number of different sources including NOX2, mitochondria and uncoupled NOS (Sag et al., 2013).

Interestingly, NOX2 expression and/or activity has also been shown to be increased in end-stage human HF in a number of studies, supporting the potential involvement of this pathway in the formation of ROS that may interfere with Ca²⁺ handling and lead to subsequent arrhythmias (Zhang M. et al., 2013). Furthermore, the elevated intracellular Na⁺ concentration seen in HF promotes the production of mitochondrial ROS (Kohlhaas et al., 2010; Viatchenko-Karpinski et al., 2014), which could ultimately lead to the potential for a vicious circle of proarrhythmic signaling via CaMKII.

Diseased hearts have been shown to have a significant increase in nNOS mRNA and protein expression (Damy et al., 2004). While on the other hand, several studies have provided evidence that NO production by eNOS is markedly diminished in HF, and an overexpression of eNOS has been shown to relieve cardiac dysfunction in a mouse model of HF (Katz et al., 1999; Jones et al., 2003; Damy et al., 2004). Under normal physiological conditions eNOS appears to decrease β-adrenergic responsiveness via inhibition of LTCC (Wang et al., 2008), therefore a reduction in this mechanism may be an additional driving force for β-adrenergic driven arrhythmias under these conditions. The overall increase in nNOS activity in HF, and the potentially altered signaling activity and targets (for example caveolae-associated molecules versus the RyR) resulting from the translocation of this molecule to the sarcolemma, may be important for deleterious Ca²⁺ handling and arrhythmia formation (Damy et al., 2004). Additionally, in HF it appears that NO production inducible NOS (iNOS) becomes of increased importance, although the role of this is currently less defined (Massion et al., 2003; Carnicer et al., 2013).

The activity of cAMP/PKA is tightly regulation by the activity of specific phosphodiesterases (PDEs) and protein phosphatases, however, the distribution of these is out of the scope of the present manuscript (see Guellich et al., 2014 for a review on this matter).

Finally, it is important to consider that the relative contribution of the different subtypes of β-ARs may also contribute to the increased arrhythmogenic phenotype observed in HF. As noted previously distinct pathways are associated with the different subtypes. Previous work has shown that the β₁ subtype of adrenoreceptors are especially downregulated in HF, while the coupling of the receptors to Gs, presumably via increased activity of the receptor kinases GRK2 and/or GRK5, is altered (Lohse et al., 2003). Interestingly, β₂-stimulation appears to be more arrhythmogenic in the failing heart when compared to the non-failing. Arrhythmogenesis appears to be driven by enhanced spontaneous SR Ca²⁺ release and aftercontractions, and is likely attributable, at least in part, to enhanced SR Ca²⁺ load secondary to PLB phosphorylation (DeSantiago et al., 2008). Away from the single cardiac myocyte, the requirement for β-Adrenergic stimulation to induce ectopic activity has also been shown in a human wedge preparation. Hearts from patients experiencing HF, exhibited ectopic beats and triggered activity in response to β₂-stimulation. The authors of this study ascribe the increase in arrhythmogenic activity due to the enhancement of transmural differences between Ca²⁺ and APD, facilitating the formation of DADs (Lang et al., 2015).

All of the data that has been discussed up till now has not considered the potential for regional differences in remodeling, which may be triggered by various stimuli, stresses and strains sensed at different anatomical locations. Taking this into account, a recent paper interestingly showed that in a porcine model of myocardial infarction and HF, regional heterogeneities in arrhythmogenic remodeling do indeed exist. In this study, it was shown that changes in multiple currents lead to a shortening of AP at the border zone of the infarct, while APs recorded from the remote zone were prolonged. This will lead to a greater dispersion of repolarization across the ventricle, which could ultimately increase the arrhythmogenic substrate. Furthermore, these authors showed that cells isolated from the remote region showed DADs with a much higher frequency than in control, and amongst those cells, nearly half also showed triggered APs. Interestingly all HF-border cells showed DADs with over half showing triggered activity often with a superimposed EAD. In addition, inhibition of CaMKII decreased the occurrence of these DADs back to control levels, further indicating the importance of this multimodal signaling molecule in arrhythmia generation in this setting (Hegyi et al., 2018b). Although in this study these DADs were not induced by β-Adrenergic stimulation, but by burst pacing, one could also postulate that regional differences will also exist in terms of β-Adrenergic responsiveness in HF. In fact, a recent abstract from the Sipido group showed that in a pig model of MI, myocytes isolated from the peri-infarct region had a higher occurrence of isoproterenol induced DADs when compared to myocytes isolated from the region remote from the infarct (Dries et al., 2018b). These data pave the way for further research in this area.

Local (Microdomain) Remodeling

So far, we have only focussed on global remodeling, however, in addition to heterogeneity seen across the ventricular wall as just described, the myocyte in itself is not homogeneous, especially when the micro-architecture of the myocyte is altered as is seen in HF. For these reasons, we must also consider local subcellular alterations. Given the improvements in imaging techniques, and experimental advances over recent years we have gained greater insights into how alterations in these ‘microdomains’ may influence arrhythmogenic outcomes in HF. Over the next paragraphs, we will discuss a number of studies that have been carried out in an attempt to elucidate how these microdomains may influence stretch- or β-adrenergic-induced arrhythmia in HF, with a view on targeted therapeutics (see the Section “Therapeutic Interventions”).

As noted previously, Ca²⁺ entry via the LTCC is the initial trigger for Ca²⁺ release from the SR, therefore it is important to discuss potential changes in this current in HF. At a global level, the majority of studies have shown that there is no alteration in whole cell Ca²⁺ current recorded from myocytes from HF patients or in animal models, although single channel studies have shown that the availability and open probability of the LTCC is increased in human HF myocytes (Beuckelmann and Erdmann, 1992; Mukherjee and Spinale, 1998; Schröder et al., 1998). However, over recent years a number of interesting observations have come to light indicating the location of LTCCs are different in HF myocytes. Alteration in the location of the LTCC will also have detrimental effects on the levels of Ca²⁺ the individual channels are exposed to. Therefore, Ca²⁺-dependent inactivation of the current as well as the dynamic modulation of the window current are likely to be altered in HF. Both of these changes will contribute to the formation of afterdepolarizations and can be influenced by β-adrenergic stimulation.

Using a rat model, combined with osmotic detubulation, Bryant et al. (2015) showed that although no differences in total I_CaL density was seen between ventricular myocytes isolated from animals that had undergone a coronary artery ligation, this lack of change resulted from differential effects at the cell surface and the T-tubules. I_CaL current density was decreased at the T-tubules while it was increased at the cell surface (Bryant et al., 2015).

An additional study from the group of Gorelik, using the super-resolution scanning patch-clamp technique showed similar findings. They elegantly showed that in both human and rat HF there was a redistribution of functional LTCCs from their physiological T-tubular location to the non-native crest of the sarcolemma. They went on to show that the open probability of these redistributed channels was dramatically increased, and the high open probability was linked to enhanced CaMKII modulation in the ‘new’ location. The current at these non-native channels resulted in an elevated I_CaL window current, which contributed to the development of EADs. This remained true when these data were fed-into a 3-dimensional left ventricle model illustrating that the phenomenon occurring at the single cell level has far reaching arrhythmogenic implications (Sanchez-Alonso et al., 2016). Interestingly, work carried out over 20 years ago indicated that there was a frequency dependent decrease in I_CaL in human dilated cardiomyopathy (Sipido et al., 1998). If this is to do with the altered LTCC microdomains, potentially due to CaMKII, remains to be seen, but should be the subject of future studies.

Localization of the LTCC to the T-tubules has previously been shown to be under control of the membrane scaffolding protein BIN1, with the knockdown of this protein leading to a reduction in surface LTCC and alterations in Ca²⁺ handling within the myocyte (Hong et al., 2010). Interestingly, BIN1 has been shown to be decreased in human HF as well as in a number of animal models, which may contribute to the alterations in patterns seen in LTCC localization (Hong et al., 2012; Caldwell et al., 2014). A more recent study has implicated that the β-adrenergic stimulation of BIN1 leads to reorganization of LTCC/RyR microdomains by also recruiting phosphorylated RyRs into the dyads. When BIN1 is downregulated, therefore, these phosphorylated RyRs may not be recruited in the dyad and arrhythmias may be promoted due to the defective Ca²⁺ handling (Fu et al., 2016).

Over 10 years ago, it was shown that in the failing heart there are a number of RyRs that become ‘orphaned’ from their LTCC counterpart in the T-tubule in spontaneously hypertensive rats (Song L.S. et al., 2006). These investigators used this model to show that this loss of coupling between the LTCC and RyR led to Ca²⁺ instability in the heart. More recently, Dries et al. (2018a) showed that in human myocytes isolated from HF patients, there were more non-coupled (to LTCC) RyRs which had more spontaneous activity than in non-HF. Hyperactivity of these non-coupled RyRs was reduced by CaMKII inhibition (Dries et al., 2018a). Previous work from the same group had illustrated that under healthy conditions, coupled (to LTCC) RyRs are distinctly modulated by CaMKII and ROS, while CaMKII and NOS1-dependent modulation of RyRs during β-adrenergic stimulation was also restricted to the dyadic cleft (Dries et al., 2013, 2016). The authors went on to use a pig model to further investigate alterations in coupled and non-coupled RyRs and their regulation in normal and pathophysiological conditions. However, after an MI, it was shown that under adrenergic stimulation using isoproterenol, Ca²⁺ waves were frequent and originated at non-coupled sites, generating larger NCX currents than in sham operated animals. Inhibition of CaMKII or mitochondrial-ROS scavenging reduced spontaneous Ca²⁺ waves, and improved excitation–contraction coupling, indicating that these could be interesting therapeutic targets (Dries et al., 2018a). A very recent paper corroborated the arrhythmogenic role of mitochondrial ROS in the formation of arrhythmias in a guinea pig model of non-ischemic HF (Dey et al., 2018). In this model, continuous telemetry recordings indicated a high frequency of premature ventricular complexes and spontaneous ventricular tachycardia/ventricular fibrillation in animals after aortic constriction and isoproterenol stimulation. Scavenging mitochondrial ROS using MitoTEMPO markedly suppressed arrhythmias as well as blunting QT prolongation and reducing QT variability. Taken all together these data indicate specific targeting of one source of ROS is adequate to reduce proarrhythmic outcomes.

In addition to alterations seen in the LTCC/RyR microdomain, and the control thereof, that may influence arrhythmogenesis we also need to consider alterations in the distribution of the different β-ARs themselves. One of the first reports investigating potential alterations was by Nikolaev et al. (2010). In that seminal study it was shown that, as opposed to the situation in healthy myocytes where β₁-adrenergic receptors are widely distributed at the cell crest and β₂-receptors (and their associated signaling pathways) are localized to the T-tubules, in HF β₂-receptors were redistributed from the transverse tubules to the cell crest, leading to a change in β₂-receptors associated compartmentation of cAMP (Nikolaev et al., 2010). These alterations lead to the β₂-receptors acting more like β₁-receptors and have detrimental effects on the cross-talk of adrenergic signaling and Ca²⁺ handling within the failing cell. Follow up studies have shown that the compartmentalization of the cAMP signaling from β₂-receptors is governed by caveolin 3, a protein that regulates the number of caveolae in the myocyte. Alteration in the T-tubule structure, levels of caveolin 3 and junctophilin 2 appear to be time-dependent, and gradually alter the β₂-signaling pathways. Furthermore, caveolin 3 overexpression in failing cells was able to restore, at least in part, the T-tubular location of the β₂-receptors (Wright et al., 2014; Schobesberger et al., 2017).

Interestingly, an additional study utilizing a rabbit model, also showed that reintroduction of caveolin-3 was able to normalizes β-adrenergic-induced contractile responses in HF myocytes, while also showing that in HF β2-induced signaling gains access to myofilament which may contribute to abnormal PKA phosphorylation of troponin I and contractile dysfunction (Barbagallo et al., 2016). In addition, work from the Sacconi group has shown that while cells from HF myocytes respond to β-adrenergic stimulation, this is not the case at the T-tubules that do not conduct APs, where the alterations seen in response may be caused by a lack of electrical activity. These data provide an alternative, or an additional, mechanism for the alterations seen in HF (Crocini et al., 2016).

Therapeutic Interventions

Despite advances in our knowledge of the pathophysiology underlying HF over the last 10 years, as outlined above, only a limited number of compounds have become available for the treatment of this debilitating disease (Gordin and Fonarow, 2016). Given the information in previous sections, we could consider targeting primary stressors (i.e., β-adrenergic stimulation or mechanical load) or downstream pathways. In Figure 3, we have summarized the most important mechanisms of afterdepolarizations, highlighting relationships between stimuli, key signaling molecules and targeted Ca²⁺ handling proteins and ion currents. Based on this scheme, we will discuss a number of (potential) interventions over the next few paragraphs that could be utilized to reduce the arrhythmogenic burden in HF patients.

FIGURE 3.

Scheme of key events in β-adrenergic and stretch signaling for the induction of afterdepolarizations as potential targets for anti-arrhythmic therapy. The central event in the generation of afterdepolarizations is a Ca²⁺ wave that can produce DAD or EAD depending on the timing of NCX activation during the cardiac cycle (diastole or systole). Mechanical stretch and β-adrenergic agonists activate protein kinases (PKA, CaMKII) and free radicals (ROS, NO) that increase RyR activity and/or SR Ca²⁺ load to produce Ca²⁺ sparks and waves. Likewise, they increase the activity of late Na⁺ and Ca²⁺ currents promoting EADs, directly via phosphorylation and redox modification, or indirectly via modulation by increased SR Ca²⁺ release secondary to enhanced SR Ca²⁺ load. Stretch-activated ion channels further destabilize membrane potential during stretch. Anti-arrhythmic strategies include targeting of upstream stressors, downstream signaling components, and Ca handling proteins or currents. Specific examples under current investigation are indicated and further discussed in the text. DAD, delayed afterdepolarizations; EAD, early afterdepolarizations; LVAD, left ventricular assistant device; NCX, Na⁺/Ca²⁺ exchanger; NO, nitric oxide; NOX, NADPH oxidase type; nNOS, neuronal nitric oxide synthase; PKA, protein kinase A; ROS, reactive oxygen species; RyR, ryanodine receptor; SACNS, stretch-activated non-selective cation currents; SR, sarcoplasmic reticulum; SERCA, SR Ca²⁺-ATPase.

Targeting Mechanical Load and Structural Remodeling

β-Blockers have been a mainstay of pharmacological treatment for HF for a number of years and are included in guidelines for treatment of HF, in part due to their antiarrhythmic properties (Funck-Brentano, 2006; Ponikowski et al., 2016). In addition to a reduction in β-adrenergic signaling that will be directly caused by β-blockers, they will also lead to a reduction of mechanical stressors that may also be responsible for the efficacy of β-blockers in preventing sudden cardiac death.

Not all β-blockers are equal, however, with some being selective at adrenoreceptor subtypes while others have additional actions at other ion channels. For instance, the antiarrhythmic effects of propranolol have also been ascribed to the potential of this compound to block Na⁺ channels (Fabritz et al., 2014). In long QT syndrome, for instance, it has been shown that propranolol has a significantly better QTc shortening effect compared to metoprolol and nadolol, and this led to a higher risk of cardiac events in symptomatic patients receiving metoprolol (Chockalingam et al., 2012). Therefore, given the information we currently have regarding the alteration in the number and location of subtypes of the β- receptors in HF, specific targeting of β-ARs, perhaps differing over the course of HF progression, may offer an interesting solution.

An additional way that mechanical load can be reduced is by the used of LVADs. Previous work has shown that the use of a LVADs together with the use of a specific pharmacological regimen, including the use of β-blockers, can lead to the sustained reversal of severe HF secondary to non-ischemic cardiomyopathy, even when the LVAD is explanted (Birks et al., 2006). Interestingly, it appeared that the alterations seen in the clinical phenotype in such patients was driven by modifications in ECC, and SR Ca²⁺ homeostasis in particular (Terracciano et al., 2004). More recent work has shown that mechanical unloading in a rat model (via heterotopic abdominal heart transplantation) reverses T-tubule remodeling, and normalizes local Ca²⁺ handling (Ibrahim et al., 2012).

Cardiac resynchronization therapy (CRT) is an alternative approach to alter mechanical loading and stretch and will assist to resynchronize ventricular wall motion. A recent meta-analysis indicated that this intervention may significantly reduce the risk of ventricular arrhythmias when compared with patients receiving ICDs. Interestingly the same study also showed that patients who were not-responsive to CRT may have had a significantly higher risk of ventricular arrhythmias (Saini et al., 2016). Furthermore, it was also shown that CRT was able to efficiently reduce subcellular heterogeneity of structure and function of RyRs and T-tubules in a canine model of HF, potentially due to the restoration of ventricular synchrony (Li et al., 2015).

Taking all of these data together it appears that alterations in mechanical load should be considered as a potential therapeutic strategy.

Another therapeutic strategy would be to potentially restrict remodeling in the first place. As previously mentioned, it appears there are a number of scaffolding proteins that are involved in the structural (and functional) remodeling of the Ca²⁺ microdomains in HF which could be the target for pharmaceutical intervention. For example, specific cardiac targeting of BIN1 could be an attractive option, although there are only limited data currently available and small molecules altering the activity of this protein are currently lacking. The potential for altering the T-tubule structure as a therapeutic intervention has recently been reviewed by Manfra et al. (2017).

Direct Targeting

It could also be possible to directly target proteins involved in arrhythmia formation under conditions of enhanced stretch or intense ß-adrenergic stimulation. For example, the potential to target stretch activated channels was recently reviewed by White (2006), however, it is perhaps of note to mention GsMTx-4, which is a peptide isolated from tarantula venom. Mixed results have been obtained with GsMTx-4 to date, with one study showing the potential for reducing the number of premature ventricular beats in an ischemia/reperfusion mouse model, while an additional study indicated no benefit in a swine model (Barrabés et al., 2015; Wang et al., 2016). Further studies are required using this compound, and derivatives of it, to truly delineate the utility of inhibition of these channels in prevention of arrhythmogenesis in HF.

Furthermore, the blockade of the components of I_TI which actually contributes to afterdepolarization could also be an interesting target. Inhibition of NCX as a target has been discussed previously (Antoons et al., 2012), however, progress in this area has been hampered the lack by selective, and/or mode selective inhibitors. More recently a number of more selective agents have been developed, including ORM-10103 which have shown their utility in preventing Ca²⁺ overload induced arrhythmias (Nagy et al., 2014). However, in a rabbit HF model NCX inhibition with ORM-10103 reduced premature ventricular beats but was unable to suppress secondary Ca²⁺ rise or the occurrence of EADs (Chang et al., 2018).

Direct inhibition of ‘leaky’ RyRs could also be an interesting target to diminish proarrhythmic Ca²⁺ leak. A recent paper showed the utility of a tetracaine-derived compound, EL20, which was able to limit arrhythmogenic Ca²⁺ waves in a CPVT model by only limiting Ca²⁺ release from RyRs associated with calmodulin (Klipp et al., 2018). Should this have utility in HF should be the subject of further investigation.

An alternative approach would be to alter the activity of SERCA in HF. A number of pre-clinical studies showed that there was anti-arrhythmic potential for increasing SERCA activity in HF using adeno-associated virus technology (Kawase et al., 2008; Lyon et al., 2011). Despite initial positive outcomes in small trials (Jessup et al., 2011), larger clinical trials have been unable to show the benefit of this approach (Greenberg et al., 2016; Hulot et al., 2017). Despite these disappointing results, further studies are still required to investigate the true utility of this approach. Activation of SERCA using small molecules may also be potentially interesting approach, and recently istaroxime has become a compound of interest (Ferrandi et al., 2013). A clinical trial using this compound is currently ongoing (ClinicalTrials.gov Identifier: NCT02617446) with the results being highly anticipated.

Targeting Downstream Modulation

An alternative avenue would be to target downstream signaling pathways that are involved. Perhaps one of the most investigated of these is the CaMKII pathway, perhaps due to the multimodal action of this kinase, and the fact that targeting this molecule may lead to selective modulation of proarrhythmic RyRs (Dries et al., 2018a). Interestingly it was recently shown that CaMKII activity persists even during chronic β-adrenergic blockade in HF, indicating that these two pathways could be targeted independently (Dewenter et al., 2017). To date, however, there are no clinically available CaMKII inhibitors, likely due to the lack of selective, bioavailable compounds. There are numerous reviews dealing with the potential of CaMKII inhibition as a potential treatment for HF, and for those reasons we will only discuss a couple of newer studies that have added fuel to the fire regarding the use of CaMKII inhibitors as antiarrhythmics (Swaminathan et al., 2012; Fischer et al., 2013; Westenbrink et al., 2013).

A recent study utilized the novel agent, GS-680, which is an ATP-dependent small molecule inhibitor of CaMKII, which appears to have good selectivity for CaMKII. In this study, this compound did not impair contractile function in failing human ventricular trabeculae and blunted the negative force-frequency relationship. Meanwhile, it increased the Ca²⁺ transient amplitude in isolated failing ventricular myocytes and reduced premature atrial contractions and afterdepolarizations in atrial cardiomyocytes (Lebek et al., 2018). Additionally, Dries et al. (2018a) recently showed that the specific CaMKII peptide inhibitor (AIP) significantly reduced the hyperactivity of RyRs in non-coupled regions without affecting spark frequency at coupled sites (Dries et al., 2018a). Finally, it has also been shown that inhibition of CaMKII is able to reduce the proarrhythmic effects of PDE-inhibitors, which act by increasing pools of cAMP (Bobin et al., 2016). If this is also true in HF should be the subject of future studies. If CaMKII inhibition will eventually function as an effective antiarrhythmic in HF still remains to be seen. While discussing CaMKII, it is also important to discuss the late Na⁺ current. Recent work has shown that inhibition of this current is able to suppress Ca²⁺ related arrhythmias by reducing CaMKII phosphorylation (Wei et al., 2017). Furthermore, ranolazine, an agent that inhibits both late Na⁺ and K⁺ channels has been shown to have antiarrhythmic effects in the intact heart in CHF and, interestingly, was not associated with drug-induced proarrhythmia (Frommeyer et al., 2012). Once again, further work is required to determine the true utility of late Na⁺ current inhibition under these circumstances.

Reactive oxygen species is one of the determinants of CaMKII activity, as well as having direct effects on Ca²⁺ handling proteins directly, so perhaps it is also possible to target ROS sources as an antiarrhythmic intervention. Interestingly the positive effect of the antioxidant vitamin C on the β-adrenergic response to dobutamine was blunted in HF patients, perhaps due to the global antioxidant properties of this agent (Mak and Newton, 2004), leading us to believe that a targeted approach may be required. The inhibition of NOX2 may be a promising approach although it may be necessary to avoid concurrent inhibition of other NADPHs which are required in other organ systems (Sag et al., 2014). Furthermore, supplementing with BH4, to ‘re-couple’ uncoupled NOS has been proposed (Moens et al., 2008), however, there have been issues with the bio-availability of this compound (see Bendall et al., 2014). The final source of ROS that could be an interesting target in mitochondrial ROS, and as noted earlier a number of studies have shown the utility of mitoTEMPO in reducing arrhythmia in HF (Dey et al., 2018; Dries et al., 2018a). The development of bioavailable safe tools to directly alter mitochondrial ROS production is still in its infancy and efforts should be concentrated to develop these.

Finally, targeting Epac has also been proposed as a potential target for arrhythmias driven by β-adrenergic stimulation, although there is discussion about its role. A recent study showed that treatment with an Epac activator, 8-CPT, enhanced the late Na⁺ current while inhibition of PKA (via PKI) did not affect the catecholamine induced increases in late Na⁺ current, indicating that Epac alone plays a crucial role in these arrhythmias (Dybkova et al., 2014; Fujita et al., 2017).

Concluding Remarks

We still have a long way to go in treating ventricular arrhythmias that occur as a result of HF, however, concentrating on various microdomains within the cardiac myocytes that are under control of multiple pathways could be of great interest. Furthermore, linking the potential electro-mechanical and mechano-electro feedback loops could aid us in treating arrhythmias that occur as a result of this debilitating disease.

Is stretch sympathetic to HF? Only time and research will fully answer this question, but in the mean time we will have to be.

Author Contributions

Both authors contributed to manuscript preparation and approved the final version of the manuscript.

Conflict of Interest Statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Abbreviations

β-AR

β-adrenergic receptor

AC

adenyl cyclase

AP

action potential

BVR

beat-to-beat variability of repolarization

CaMKII

Ca²⁺/calmodulin dependent protein kinase II

cAMP

cyclic adenosine 3′,5′-monophosphate

Casq2

cardiac calsequestrin

CICR

calcium induced calcium release

DAD

delayed afterdepolarization

EAD

early afterdepolarization

ECC

excitation contraction coupling

Epac

exchange protein activated by cAMP

HF

heart failure

I_TI

transient inward current

LTCC

L-type calcium channel

LVAD

left ventricular assist device

NCX

sodium/calcium exchanger

NO

nitric oxide

NOS

nitric oxide synthase

NOX2

NADPH oxidase 2

PI3K

phosphoinositide 3-kinase

PKA

protein kinase A

PLB

phospholamban

ROS

reactive oxygen species

RyR

ryanodine receptor

SACNS

stretch activated non-selective cation current

SERCA

SR Ca²⁺-ATPase

SR

sarcoplasmic reticulum

INaL

late sodium current