Figure 1.

HFD leads to increased T-cell number and clonality. A and B: AT CD8⁺ number was increased in HFD mice (two-tailed t test) (A), whereas CD4⁺ T cells were not significantly increased (B). C: There was no difference in the number of liver CD8⁺ T cells. D: The TCR repertoires of AT CD8⁺ T cells of HFD-fed mice are significantly more clonal than those of LFD-fed mice (P < 0.05, two-tailed Student t test). E: AT CD4⁺ clonality did not differ between HFD and LFD (P = 0.18, two-tailed Student t test). F: Liver CD8⁺ T cells of HFD-fed mice are not significantly clonal compared with LFD-fed mice (P = 0.18, two-tailed Student t test). Data points represent the clonality of each mouse as calculated using normalized Shannon entropy. Data are mean ± SEM (n = 4–5 mice/group). *P ≤ 0.05.