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. 2018 Aug;7(2):138–147. doi: 10.2174/2211536607666180416152112

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© 2018 Bentham Science Publishers

This is an open access article licensed under the terms of the Creative Commons Attribution-Non-Commercial 4.0 International Public License (CC BY-NC 4.0) (https://creativecommons.org/licenses/by-nc/4.0/legalcode), which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.

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Fig. (3) — Heat/Freeze (H/F) cycle significantly improves miRNA detection. (A) Comparison of array results from two independent samples from the same subject after H/F process. Venn diagram between the two samples is shown in the top panel and regression analysis in the bottom panel (based on Ct values). (B) Comparison of array results from H/F and unprocessed serum samples. Venn diagram of two unprocessed samples and two H/F samples from the same subject is shown in the top panel. Regression analysis between one unprocessed serum sample and one H/F sample is shown in the bottom panel (based on Ct values). (C) Comparison of array results from a Qiagen RNA prep and Heat/Freeze (H/F) cycled serum. Top panel depicts miRNAs detected in all samples. Regression analysis between one RNA-extracted sample and one H/F processed serum sample (based on Ct values).