FIG 2.

Regulation of genes downstream of class I (GEMM) riboswitches by c-di-GMP. C. difficile with vector or pDccA was grown with a range of nisin concentrations (µg/ml). Cultures were divided for quantification of intracellular c-di-GMP concentration by LC-MS (red) or measurement of transcript abundance for the downstream open reading frame by qRT-PCR (blue). Lines and error bars represent the geometric mean and geometric standard deviation. Not shown are those for which no downstream open reading frame or transcript was identified.