FIG 4.

Adenine-dependent activation by LexA-Grf10 does not require Bas1. (A) Strains RAC201, RAC216, RAC290, and RAC291, with their relevant genotypes indicated, were assessed for adenine prototrophy on SC medium containing or lack adenine, as indicated. Plates were incubated at 30°C for 33 h. (B) Strains RAC201, RAC287, RAC216, and RAC290 were grown and diluted as described in Fig. 2 and plated on the media as indicated. These experiments were performed with at least three independent transformants.