Table 3. Maximum Surface Coverage (% of Full Hexagonal Packing) for Different Lipid Compositions along with Maximum Vesicle Thickness (Calculated from SPR Fresnel-Layer Modeling), Maximum Coupled Water Mass and Water-to-Lipid ratio at the Point of Maximum Coupled Water Mass (Calculated from Eq 2 Using SPR and QCM-Z Data)a.
| lipid | αmax(%) | dmax (nm) | Γmax,H2O (ng/cm2) | water-to-lipid ratio at Γmax,H2O |
|---|---|---|---|---|
| DOPC | 8.7 ± 1.0 | 13.2 ± 0.6 | 588 ± 81 | 5.05 ± 1.17 |
| DOPC–DOPS | 13.3 ± 0.3 | 15.3 ± 2.3 | 726 ± 7 | 4.86 ± 0.08 |
| POPC | 12.3 ± 0.2 | 21.2 ± 2.3 | 754 ± 46 | 4.00 ± 0.56 |
| DOPC–Sm–Chol | 11.1 ± 1.8 | 16.7 ± 3.8 | 817 ± 22 | 4.07 ± 0.13 |
| DPPC | 71.2 ± 2.2 | 81.9 ± 3.3 | 2644 ± 188 | 1.96 ± 0.16 |
a
QCM-Z frequency values used in the analysis were calculated using the Sauerbrey equation (Supporting Information eq S3).