Fig. 5.

Histological assessment of the vasculature of C6 DDAH ± DOX (group A) and D27 xenografts. a Representative composite images from frozen whole C6 DDAH ± DOX tumour sections stained with the perfusion marker Hoechst 33342 (blue fluorescence) and the hypoxia marker pimonidazole (green fluorescence). b H&E-stained frozen sections from C6 DDAH ± DOX and D27 tumours indicating necrotic regions (arrows). c Composite images from frozen C6 DDAH ± DOX and D27 tumour sections stained with the endothelial cell marker CD31 (green fluorescence) and the perivascular cell marker α-SMA (red fluorescence). Scale bar is 1 mm