Table 2.
Primer sequences used for (q)PCR
| Gene | Sense primer sequence | Antisense primer sequence |
|---|---|---|
| Fzd1 | GCCCTCCTACCTCAACTACCA | ACTGACCAAATGCCAATCCA |
| Fzd2 | GCTTCCACCTTCTTCACTGTC | GCAGCCCTCCTTCTTGGT |
| Fzd3 | CTTCCCTGTCGTAGGCTGTGT | GGGCTCCTTCAGTTGGTTCT |
| Fzd4 | ATGAACTGACTGGCTTGTGCT | TGTCTTTGTCCCATCCTTTTG |
| Fzd5 | TACCCAGCCTGTCGCTAAAC | AAAACCGTCCAAAGATAAACTGC |
| Fzd6 | GCGGAGTGAAGGAAGGATTAG | TGAACAAGCAGAGATGTGGAA |
| Fzd7 | CGCCTCTGTTCGTCTACCTCT | CTTGGTGCCGTCGTGTTT |
| Fzd8 | GCCTATGGTGAGCGTGTCC | CTGGCTGAAAAAGGGGTTGT |
| Fzd9 | CTGGTGCTGGGCAGTAGTTT | GCCAGAAGTCCATGTTGAGG |
| Fzd10 | CCTTCATCCTCTCGGGCTTC | AGGCGTTCGTAAAAGTAGCAG |
| Wnt1 | CAACAGCAGTGGCCGATGGTGG | CGGCCTGCCTCGTTGTTGTGAAG |
| Wnt2 | GTCATGAACCAGGATGGCACA | TGTGTGCACATCCAGAGCTTC |
| Wnt2b | AAGATGGTGCCAACTTCACCG | CTGCCTTCTTGGGGGCTTTGC |
| Wnt3 | GAGAGCCTCCCCGTCCACAG | CTGCCAGGAGTGTATTCGCATC |
| Wnt3a | CAGGAACTACGTGGAGATCATG | CCATCCCACCAAACTCGATGTC |
| Wnt4 | GCTCTGACAACATCGCCTAC | CTTCTCTCCCGCACATCC |
| Wnt5a | GACCTGGTCTACATCGACCCC | GCAGCACCAGTGGAACTTGCA |
| Wnt5b | TGAAGGAGAAGTACGACAGC | CTCTTGAACTGGTTGTAGCC |
| Wnt6 | TTATGGACCCTACCAGCAT | ATGTCCTGTTGCAGGATG |
| Wnt7a | GCCGTTCACGTGGAGCCTGTGCGTGC | AGCATCCTGCCAGGGAGCCCGCAGCT |
| Wnt7b | GATTCGGCCGCTGGAACTGCTC | TGGCCCACCTCGCGGAACTTAG |
| Wnt8a | CTGGTCAGTGAACAATTTCC | GTAGCACTTCTCAGCCTGTT |
| Wnt8b | GTCTTTTCACCTGTGTCCTC | AGGCTGCAGTTTCTAGTCAG |
| Wnt10a | CTGTTCTTCCTACTGCTGCT | ACACACACCTCCATCTGC |
| Wnt10b | GCACCACAGCGCCATCCTCAAG | GGGGTCTCGCTCACAGAAGTCAGGA |
| Wnt11 | CACTGAACCAGACGCAACAC | CCTCTCTCCAGGTCAAGCAAA |
| Wnt14 | ACAAGTATGAGACGGCACTC | AGAAGCTAGGCGAGTCATC |
| Wnt15 | TGAAACTGCGCTATGACTC | GTGAGTCCTCCATGTACACC |
| Wnt16 | GAGAGATGGAACTGCATGAT | GATGGGGAAATCTAGGAACT |
| Axin2 | TTGAATGAAGAAGAGGAGTGGA | TCGGGAAATGAGGTAGAGACA |
| Ccnd1 | GTCCATGCGGAAGATCGTCG | TCTCCTTCATCTTAGAGGCCACG |
| C-Myc | CACAGCAAACCTCCTCACAG | CGCCTCTTGACATTCTCCTC |
| Angpt1 | GCTGAACGGTCACACAGAGA | CTTTCCCCCTCAAAGAAAGC |
| Angpt2 | TTATCACAGCACCAGCAAGC | TTCGCGAGAACAAATGTGAG |
| VEGFa | AAGGAGGAGGGCAGAATCAT | ATCTGCATGGTGATGTTGGA |
| VEGFr2 | AGCGATGGCCTCTTCTGTAA | ACACGACTCCATGTTGGTCA |
| Flt1 | TGTCAATGTGAAACCCCAGA | GTCACACCTTGCTCCGGAAT |
| DSCR1 | GAGGACGCATTCCAAATCAT | AGTCCCAAATGTCCTTGTGC |
| TF | TACTTGGCACGGGTCTTCTC | TGTCCGAGGTTTGTCTCCA |
| Ets1 | GGAGCAGCCAGTCATCTTTC | GGTCCCGCACATAGTCCTT |
| PKCα | CGACTGGGAAAAACTGGAGA | ACTGGGGGTTGACATACGAG |
| PKCδ | ATTGCCGACTTTGGGATGT | TGAAGAAGGGGTGGATTTTG |
| PKCε | AAGCCACCCTTCAAACCAC | GGCATCAGGTCTTCACCAAA |
| PKCη | TCCCACACAAGTTCAGCATC | CCCAATCCCATTTCCTTCTT |
| MMP1 | GATTCGGGGAGAAGTGATGTT | CGGGTAGAAGGGATTTGTG |
| Β-actin | TCCCTGGAGAAGAGCTACGA | AGCACTGTGTTGGCGTACAG |