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. 2018 Sep 13;34(40):12083–12092. doi: 10.1021/acs.langmuir.8b01272

Figure 3.

Figure 3

Normalized fluorescence intensities of the mTurquoise2 and SYFP2 FRET pair in C3Ms at different FRET pair amounts (10 to 100%: FRET pair/SBFP2 ratio) upon donor excitation (440 nm). The total protein concentration was kept at 1 μM using SBFP2 to replace both mTurquoise2 and SYFP2 to keep the FRET pair composition constant at 1:1 (mTurquoise2/SYFP2) and change the FRET pair amount in the C3Ms. The concentration of P2MVP128-b-PEO477 was 0.18 μM. (A) Fluorescence emission spectra at different FRET pair amounts (normalized at 517 nm). (B) FRET efficiency as a function of the FRET pair amount. The solid line is a guide to the eye.