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. 2018 Oct 30;9(5):e01871-18. doi: 10.1128/mBio.01871-18

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Copyright © 2018 Collins et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution 4.0 International license.

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FIG 2 — Introduction of structural and NS Asibi genes into the 17D backbone alters nucleotide diversity of IC-derived 17D-204 virus. (A) Structural chimeric viruses. (B and C) NS mutant viruses. The nucleotide diversity of IC-derived Asibi and 17D-204 viruses is statistically diverse in all genes except capsid and pre-membrane as determined by Mann-Whitney test. The nucleotide diversity of prME chimeric and NS2B-109 and NS4B-95 mutant viruses is statistically more diverse in multiple genes compared to IC-derived 17D-204 virus. The nucleotide diversity of prME chimeric virus was the only Asibi backbone virus that was statistically less diverse in multiple genes compared to IC-derived Asibi virus. Chimeric and mutant viruses were compared to parental IC-derived viruses using Kruskal-Wallis test, followed by Dunn’s comparison.