Figure 3.

Anemic mice treated with Sucrosomial^® Iron and Ferrous Sulfate showed increased body iron stores. (A) Liver and (D) spleen iron contents were quantified in all groups of mice treated with Saline, Vehicle, Sucrosomial^® Iron and Ferrous Fulfate (1 mg/kg) by gavage for two weeks. (B) and (E) ELISA assay for the quantification of l-ferritin in liver and spleen. In (A), (B), (D) and (E) Saline (circles), Vehicle (squares), Sucrosomial^® Iron (triangles) and Ferrous Sulfate (diamonds).; (C) and (F) Representative image of Prussian blue stain plus DAB enhancement of ferritin-iron (FT-Iron) (top) and representative image of Western blotting for l-ferritin in liver and spleen of treated mice. The densitometry was performed using ImageJ. The values (N = 3) were normalized to GAPDH or actin for liver and spleen respectively. p values were obtained by one-way ANOVA.