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. 2018 Oct 2;10(10):1399. doi: 10.3390/nu10101399

Table 1.

Sequences of primers used for quantitative real-time polymerase chain reaction (PCR).

Target Gene Primer Sequence (5′-3′)
Forward Reverse
hBD-2 5′-GGTGTTTTTGGTGGTATAGGC-3′ 5′-AGGGCAAAAGACTGGATGACA-3′
hCXCL1 5′-AATCCTGCATCCCCCATA-3′ 5′-TGTCTCTCTTTCCTCTTCTGTTCCT-3′
hCXCL5 5′-TGCGTTGCGTTTGTTTACAG-3′ 5′-GAAAAGGGGCTTCTGGATCA-3′
hCXCL8 (IL-8) 5′-ATGACTTCCAAGCTGGCCGTG-3′ 5′-TTATGAATTCTCAGCCCTCTTCAAAAACTTCTC-3′
hHPRT 5′-TATGGCGACCCGCAGCCCT-3′ 5′-CATCTCGAGCAAGACGTTCAG-3′
hS100A8 5′-GGGAATTTCCATGCCGTCT-3′ 5′-CCTTTTTCCTGATATACTGAGGAC-3′
hS100A9 5′-CAGCTGGAACGCAACATAGA-3′ 5′-TCAGCTGCTTGTCTGCATTT-3′
mCXCL1 5′-CACCTCAAGAACATCCAGAGCT-3′ 5′-CAAGCAGAACTGAACTACCATCG-3′
mDEFB4 5′-GCTTCAGTCATGAGGATCCAT-3′ 5′-CTTGCTGGTTCTTCGTCTTTT-3′
mHPRT 5′-CACAGGACTAGAACACCTGC-3′ 5′-GCTGGTGAAAAGGACCTCT-3′
mCXCL2 5′-TTGCCTTGACCCTGAAGCCCCC-3′ 5′-GGCACATCAGGTACGATCCAGGC-3′
mS100A8 5′-AAATCACCATGCCCTCTACAAG-3′ 5′-CCCACTTTTATCACCATCGCAA-3′
mS100A9 5′-ATACTCTAGGAAGGAAGGACACC-3′ 5′-TCCATGATGTCATTTATGAGGGC-3′

BD-2, beta-defensin 2; CXCL: C-X-C motif ligand; IL, interleukin, HPRT, hypoxanthine-guanine phosphoribosyltransferase; S100, S100 calcium binding protein; DEFB4, defensin beta 4; h, human; m, mouse; F, forward, R, reverse.