Figure 4.

⁶⁸Ga-sCD146 targeted AMOT protein. (A) ⁶⁸Ga-sCD146 specificity assessed by autoradiography on sections of gastrocnemius muscle on day 5 post-ischaemia, after incubation with 10-15 kBq of ⁶⁸Ga-sCD146 (2.05 nM in PBS) for 30 min, without or after 60 min of C-18 anti-AMOT antibody blocking (1 μM) (***P = 0.0008; n = 8). (B) Representative PET images of ⁶⁸Ga-sCD146 acquired 40 min post-injection, without (a) and after blocking experiments (b) with a 100-fold excess of unradiolabelled peptide injected 30 min before the radiotracer injection (white arrow: ischaemic hindlimb). (C) Hindlimb uptake of ⁶⁸Ga-sCD146 quantification expressed as ischaemic-to-contralateral muscle ratio on day 5 post-surgery and 40 min post-injection. ⁶⁸Ga-sCD146 uptake was significantly reduced in the presence of unlabelled peptide sCD146 (160 μg). (*P = 0.03; n = 3). (D) Correlation between AMOT expression assessed by immunohistochemistry and ⁶⁸Ga-sCD146 uptake by PET imaging at all acquisition times. (P = 0.02; n = 5).