Figure 3.

Experimental process. (A) each bacteria strain tested was striked on an agar plate containing Kanamycin, and incubated overnight at 37 °C; (B) a starter was grown from a single colony from the striked plate, and incubated overnight at 37 °C in a shaking incubator; (C) the starter was refreshed by adding 200 μL of the overnight culture into 10 mL of fresh LB, and then grown for 3–4 h at 30 °C in a non-shaking incubator; (D) the bacteria strains were then exposed to the different samples of different concentrations in a high-throughput measurement using a 96-well plate; (E,F) the toxicity (Relative Light Unit (RLU)) and growth (O.D. 600 nm) signals were measured continuously during the 16 h incubation at 26 °C, in the Luminometer and TECAN reader, respectively.