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. 2018 Aug 10;23(8):2004. doi: 10.3390/molecules23082004

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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mRNA analysis of adipogenic and lipogenic factors. The mRNA levels of two adipogenic transcription factors (PPARγ (A) and C/EBPα (B)) and two lipogenic proteins (aP2 (C) and FAS (D)) genes were measured by RT-PCR in the MDI + morusin-treated 3T3-L1 adipocytes using specific primers. The data represents the means ± SD of three replicates. * indicates p < 0.05 compared to the untreated group. ^# indicates p < 0.05 compared to the MDI + vehicle-treated group. OT; orlistat, MDI; adipogenic cocktail consisting of 3-isobutyl-1-methylxanthine, dexamethasone, and insulin.