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. 2018 Oct 8;23(10):2565. doi: 10.3390/molecules23102565

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© 2018 by the authors.

Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).

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Encapsulation efficiency (EE) of adenovirus into γ-PGA-chitosan NPs; (a) EE evaluated by measuring the difference of Ad infectivity in the supernatant and the control. Difference refers to the amount of encapsulated Ad inside the particle, which means the pellet after centrifugation containing Ad. (b) HEK293A cells infected with supernatant containing Ad from the same dilution of the control and NP suspension. Black dots represent cells infected with Ad, and titers were calculated according to the Adeno-X Rapid Titter Kit manufacturer instructions. Magnification power ×200, scale bar 100 µm. Experiments were conducted in triplicate (n = 3).

Encapsulation efficiency (EE) of adenovirus into γ-PGA-chitosan NPs; (a) EE evaluated by measuring the difference of Ad infectivity in the supernatant and the control. Difference refers to the amount of encapsulated Ad inside the particle, which means the pellet after centrifugation containing Ad. (b) HEK293A cells infected with supernatant containing Ad from the same dilution of the control and NP suspension. Black dots represent cells infected with Ad, and titers were calculated according to the Adeno-X Rapid Titter Kit manufacturer instructions. Magnification power ×200, scale bar 100 µm. Experiments were conducted in triplicate (n = 3).