Table 4.
Extraction recovery and matrix effect of the marker constituents.
| Nominal Conc. (ng/mL) | R 1 | R 2 | R 3 | ME (%) 1 | RE (%) 2 |
|---|---|---|---|---|---|
| Scoparone | |||||
| 10 | 44,912 ± 1152 | 48,721 ± 2536 | 41,883 ± 1236 | 113.0 ± 16.7 | 99.8 ± 13.1 |
| 100 | 427,813 ± 14,929 | 478,693 ± 2688 | 424,339 ± 15,638 | 109.5 ± 2.0 | 99.5 ± 3.5 |
| 1000 | 4,400,014 ± 56,880 | 4,332,780 ± 178,478 | 3,636,719 ± 220,707 | 97.5 ± 1.5 | 99.7 ± 5.2 |
| mean ± SD | 106.7 ± 6.7 | 99.7 ± 7.2 | |||
| Geniposide | |||||
| 10 | 4690 ± 482 | 7474 ± 582 | 5863 ± 412 | 167.7 ± 34.5 | 91.2 ± 10.6 |
| 100 | 49,536 ± 2178 | 67,386 ± 1831 | 54,524 ± 2940 | 133.5 ± 11.3 | 90.9 ± 7.1 |
| 1000 | 649,073 ± 7233 | 771,802 ± 15,246 | 607,190 ± 24,396 | 117.9 ± 5.9 | 93.5 ± 3.5 |
| mean ± SD | 139.7 ± 17.2 | 91.9 ± 7.1 | |||
| Rhein | |||||
| 50 | 15,161 ± 597 | 12,484 ± 309 | 12,858 ± 653 | 85.1 ± 5.2 | 121.5 ± 11.1 |
| 100 | 27,608 ± 879 | 24,708 ± 1230 | 23,412 ± 1000 | 87.6 ± 3.7 | 106.5 ± 5.8 |
| 1000 | 330,092 ± 2490 | 285,450 ± 3193 | 241,434 ± 977 | 85.8 ± 5.1 | 100.5 ± 2.0 |
| mean ± SD | 86.2 ± 4.7 | 109.5 ± 6.3 | |||
| Carbamazepine (IS) | |||||
| 10 | 289,244 ± 6018 | 282,335 ± 3741 | 244,125 ± 3117 | 99.9 ± 2.9 | 85.7 ± 5.2 |
Data are expressed as the mean ± S.D. (n = 3). 1 ME: Matrix effect (%) calculated as (R 2/R 1) × 100%. 2 RE: Recovery (%) calculated as (R 3/R 2) × 100%.