Table 5.
Gradient for separation of HMO by hydrophilic interaction liquid chromatography with fluorescence detection (HILIC-FLD).
| Time (min) | Flow (mL/min) | %A | %B | Valve |
|---|---|---|---|---|
| 0 | 0.6 | 98 | 2 | waste |
| 2.5 | 0.6 | 98 | 2 | analyse |
| 3.0 | 0.6 | 88 | 12 | analyse |
| 10.0 | 0.6 | 88 | 12 | analyse |
| 20 | 0.6 | 83 | 17 | analyse |
| 33.0 | 0.6 | 83 | 17 | analyse |
| 34.0 | 0.45 | 30 | 70 | analyse |
| 37.0 | 0.45 | 30 | 70 | analyse |
| 39.0 | 0.45 | 90 | 10 | analyse |
| 45.0 | 0.6 | 90 | 10 | analyse |
| 45.1 | 0.6 | 98 | 2 | waste |
| 46 | 0.6 | 98 | 2 | waste |
Eluent A = acetonitrile; eluent B = ammonium formate (100 mmol/L, pH 4.4).