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Visualization of Z-RNA and Zα-EGFP protein. Lane 1: Z-RNA only, Lane 2: RNA + Zα-EGFP, Lane 3: Zα-EGFP only. The different modes are indicated in upper. [RNA] = 1 μM, [Zα-EGFP] = 10 μM, [NaCl] = 100 mM, [Tris-HCl (pH7.0)] = 10 mM, [DTT] = 5 mM, 10% glycerol, 10 μg/mL BSA. Z-RNA: r(CGCGUGCG)-Cy3/r(C[m8Gm]CAC[m8Gm]CG).
