Table 5.
Primers used in this work.
| Primer | Sequence (5′ → 3′) | Description or Reference |
|---|---|---|
| P1 | TCCCCCGGGCAAGCCTAGCCATTCCTC (Smal I) | P1 to P4, primers for ilvE deletion |
| P2 | GCTCTAGACGTCTACCAGCAGTTCAAG (Xba I) | |
| P3 | GCTCTAGATGGGATACGAAGTAGAAGAGC (Xba I) | |
| P4 | ACGCGTCGACTTTCCAACCGTCAGCTG (Sal I) | |
| P5 | ATGACGTCATTAGAGTTCA | P5 and P6: primers for ilvE deletion identification |
| P6 | GGTCTTAAAACCGGTTGAT | |
| P7 | GGGGTACCATGAGTTCAGTTTCGCTGC (Kpn I) | P7 and P8: primers for aspB and aspB deletion identification |
| P8 | GCTCTAGATCTCCGCTGTATTCACTTTTAG (Xba I) | |
| P9 | GGAATTCTATCTTGTGAACTCCCCCAG (EcoR I) | P9 to P12, primers for aspB deletion |
| P10 | ACGCGTCGACTATCAACGATGCCATCCAG (Sal I) | |
| P11 | ACGCGTCGACCCGAAGTTCAACAAGGTTCTG (Sal I) | |
| P12 | CCCAAGCTTGGCCAGGCTCAAAATCTC (Hind III) | |
| P13 | GGAATTCTGGAGAACCATCGCATGTTTC (EcoR I) | P13 and P14: primers for tyrB |
| P14 | CGGGATCCTAATTTCACTGCAGGCTGGG (BamH I) | |
| P15 | GGAATTCATGAATAAGCTTATTGAACGAG (EcoR I) | P15 and P16: primers for ybgE |
| P16 | CGGGATCCTCACACTTCCACTGTCCAG (BamH I) | |
| P17 | GGAATTCCAGCGTTAATCTACTCATCATG (EcoR I) | P17 and P18: primers for CaIlvE |
| P18 | CGGGATCCTTTGCAACAGCCCATTC (BamH I) | |
| P19 | GGAATTCATGAATAAGCTTATTGAACGAG (EcoR I) | P19 and P20: primers for aspC |
| P20 | CGGGATCCTTACAGCACTGCCACAATCG (BamH I) | |
| P21 | GGAATTCATGAAATTGGCTGGGTTATC (EcoR I) | P21 and P22: primers for yhdR |
| P22 | CGGGATCCTGGATTGGAAGAGGAAGG (BamH I) | |
| P23 | GGGGTACCATGGAAATAACACCGTCC (Kpn I) | P23 and P24: primers for ywfG |
| P24 | GCTCTAGATTAGCGGGATGTTTCTTG (Xba I) |
Underlining shows the restriction site for the enzyme indicated in parentheses.