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Coupling of the peptides to DOTA. Scheme for the synthesis of the two contrast agents (CA), namely Gd-DOTA-P88 and Gd-DOTA-Scramble, which were produced by coupling P88 or Scramble to DOTA, and thereafter complexing it with GdCl3 to prepare the CA for MRI. The characteristics of the chemical structures are outlined beneath the reaction.
